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Two-photon lithography (TPL) is a versatile technology for additive manufacturing of 2D and 3D micro/nanostructures with sub-wavelength resolved features. Recent advancement in laser technology has enabled the application of TPL fabricated structures in several fields such as microelectronics, photonics, optoelectronics, microfluidics, and plasmonic devices. However, the lack of two-photon polymerizable resins (TPPRs) induces bottleneck to the growth of TPL to its true potential, and hence continuous research efforts are focused on developing efficient TPPRs. In this article, we review the recent advancements in PI and TPPR formulation and the impact of process parameters on fabrication of 2D and 3D structures for specific applications. The fundamentals of TPL are described, followed by techniques used for achieving improved resolution and functional micro/nanostructures. Finally, a critical outlook and future prospects of TPPR formulation for specific applications are presented.
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http://dx.doi.org/10.1016/j.isci.2023.106374 | DOI Listing |
Adv Healthc Mater
September 2025
Department of Mechanical Engineering, University of Arkansas, Fayetteville, AR, 72701, USA.
3D scaffold architecture is critical for directing human neural stem cell (hNSC) fate and spatial organization. In this study, two-photon lithography (TPL) is used to fabricate microcapillary scaffolds based on the Hilbert space-filling curve as biomimetic basement membrane structures for guiding hippocampal-derived hNSC differentiation. The scaffolds feature 80 µm lumens with porous ellipsoidal membranes suspended above the substrate to provide topographical cues and permit nutrient diffusion while maintaining mechanical stability.
View Article and Find Full Text PDFSmall
September 2025
Department of Chemical and Biological Engineering, University of Colorado Boulder, Boulder, CO, 80303, USA.
Adoptive cell transfers (ACTs) can interact specifically with inflamed tissues, but lack a mechanism for transport through viscous biological barriers such as mucus when administered locally. Further, maintaining cell function is challenging due to the loss of cellular phenotypes in diseased microenvironments. In this work, the use of magnetically controlled helical microrobots is examined to transport macrophages through physiologically representative mucus and maintain functional phenotypes through drug elution for improved cell delivery.
View Article and Find Full Text PDFConventional digital cameras combine absorbing color filter arrays with microlenses to achieve color imaging and improve efficiency. Such cameras require multi-step and multi-material fabrication processes. Several recent efforts have investigated metasurface-based color routing to combine focusing with filtering in a single functional layer with an improved efficiency.
View Article and Find Full Text PDFAdv Mater
August 2025
Department of Biomedical Engineering and Institute for Quantitative Health Science and Engineering, Michigan State University, East Lansing, MI, 48824, USA.
Microrobots hold immense potential in biomedical applications, including drug delivery, disease diagnostics, and minimally invasive surgeries. However, two key challenges hinder their clinical translation: achieving scalable and precision fabrication, and enabling non-invasive imaging and tracking within deep biological tissues. Magnetic particle imaging (MPI), a cutting-edge imaging modality, addresses these challenges by detecting the magnetization of nanoparticles and visualizing superparamagnetic nanoparticles (SPIONs) with sub-millimeter resolution, free from interference by biological tissues.
View Article and Find Full Text PDFAdv Mater
August 2025
Laboratory of Process Engineering, NeptunLab, Department of Microsystems Engineering (IMTEK), University of Freiburg, 79110, Freiburg, Germany.
Glasses are utilized for their outstanding optical, mechanical, and thermal properties. However, conventional production methods mostly yield in glasses with uniform compositions and material properties. Here a novel lithographic approach is presented for high-resolution 3D dopant integration at defined positions, which enables property modifications in specific regions.
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