Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Microvascular basement membrane (BM) plays a pivotal role in the interactions of astrocyte with endothelium to maintain the blood-brain barrier (BBB) homeostasis; however, the significance and precise regulation of the endothelial cell-derived BM component in the BBB remain incompletely understood. Here, we report that conditional knockout of Atg7 in endothelial cells (Atg7-ECKO) leads to astrocyte-microvascular disassociation in the brain. Our results reveal astrocytic endfeet detachment from microvessels and BBB leakage in Atg7-ECKO mice. Furthermore, we find that the absence of endothelial Atg7 downregulates the expression of fibronectin, a major BM component of the BBB, causing significantly reduced coverage of astrocytes along cerebral microvessels. We reveal Atg7 triggers the expression of endothelial fibronectin via regulating PKA activity to affect the phosphorylation of cAMP-responsive element-binding protein. These results suggest that Atg7-regulated endothelial fibronectin production is required for astrocytes adhesion to microvascular wall for maintaining the BBB homeostasis. Thus, endothelial Atg7 plays an essential role in astrocyte-endothelium interactions to maintain the BBB integrity.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10067974 | PMC |
| http://dx.doi.org/10.1083/jcb.202103098 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Oxidative Stress Disrupts Gill Function in : Consequences for Ion Transport, Apoptosis, and Autophagy.
Antioxidants (Basel)
July 2025
Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China.
Oxidative stress is a key mediator of physiological dysfunction in aquatic organisms under environmental challenges, yet its comprehensive impacts on gill physiology require further clarification. This study investigated the molecular and cellular responses of gills to hydrogen peroxide (HO)-induced oxidative stress, integrating antioxidant defense, ion transport regulation, and stress-induced cell apoptosis and autophagy. Morphological alterations in the gill filaments were observed, characterized by septum degeneration, accumulation of haemolymph cells, and pronounced swelling.
View Article and Find Full Text PDFActivation of mitophagy and proteasomal degradation confers resistance to developmental defects in postnatal skeletal muscle.
J Biomed Sci
August 2025
Department of Kinesiology and Health Sciences, University of Waterloo, 200 University Ave. West, Waterloo, ON, N2L 3G1, Canada.
Background: Postnatal skeletal muscle development leads to increased muscle mass, strength, and mitochondrial function, but the role of mitochondrial remodeling during this period is unclear. This study investigates mitochondrial remodeling during postnatal muscle development and examines how constitutive autophagy deficiency impacts these processes.
Methods: We initially performed a broad RNA-Seq analysis using a publicly available GEO database of skeletal muscle from postnatal day 7 (P7) to postnatal day 112 (P112) to identify differentially expressed genes.
Tetrahydromagnolol induces autophagic cell death by targeting the mA reader protein YTHDF2 and enhances the efficacy of anti-PD-1 immunotherapy in pancreatic cancer cells.
Theranostics
August 2025
School of Pharmacy, Hangzhou Normal University, Hangzhou, Zhejiang, China.
Tetrahydromagnolol (THM) is a bioactive compound derived from . Although other compounds from this plant, such as magnolol and honokiol, have shown significant anticancer potential, the anticancer activities of THM remain unreported. This study aims to investigate the anticancer effects and underlying molecular mechanisms of THM in pancreatic cancer cells.
View Article and Find Full Text PDFTriflumizole induces excessive reactive oxygen species, inhibits the GPR30/PI3K/AKT pathway, and triggers apoptosis and autophagy in GC-1 spermatogonia cells.
Pestic Biochem Physiol
September 2025
Department of Animal Science and Technology, Chung-Ang University, Anseong, Gyeonggi-Do 17546, Republic of Korea. Electronic address:
Triflumizole (TFZ) is a widely-used imidazole-based fungicide effective against a broad range of fungal infections. Although its obesogenic and endocrine-disrupting properties have been studied, the cytotoxic effects of TFZ on male reproductive cells and the underlying mechanisms remain poorly understood. This study demonstrates that TFZ inhibits GC-1 spermatogonia (spg) cell proliferation, a type B spermatogonia, with an IC of 30.
View Article and Find Full Text PDFDysfunctional autophagy triggers STING1 activation to exacerbate cartilage degeneration in obesity-associated osteoarthritis.
Autophagy
August 2025
Center for Translational Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, P.R. China.
Obesity, a major risk factor for osteoarthritis (OA), is related to increased circulating levels of free fatty acids (FFAs). However, the molecular mechanism underlying this metabolic OA phenotype remains unknown. We found that mice fed a high-fat diet (HFD) became obese and developed OA in their knee joints.
View Article and Find Full Text PDF