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Article Abstract
Free cyanide is a hazardous pollutant released from steel industries. Environmentally-safe remediation of cyanide-contaminated wastewater is required. In this work, Pseudomonas stutzeri (ASNBRI_B12), Trichoderma longibrachiatum (ASNBRI_F9), Trichoderma saturnisporum (ASNBRI_F10) and Trichoderma citrinoviride (ASNBRI_F14) were isolated from blast-furnace wastewater and activated-sludge by enrichment culture. Elevated microbial growth, rhodanese activity (82 %) and GSSG (128 %) were observed with 20 mg-CN L. Cyanide degradation > 99 % on 3rd d as evaluated through ion chromatography, followed by first-order kinetics (r = 0.94-0.99). Cyanide degradation in wastewater (20 mg-CN L, pH 6.5) was studied in ASNBRI_F10 and ASNBRI_F14 which displayed increased biomass to 49.7 % and 21.6 % respectively. Maximum cyanide degradation of 99.9 % in 48 h was shown by an immobilized consortium of ASNBRI_F10 and ASNBRI_F14. FTIR analysis revealed that cyanide treatment alters functional groups on microbial cell walls. The novel consortium of T. saturnisporum-T. citrinoviride in the form of immobilized culture can be employed to treat cyanide-contaminated wastewater.
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| http://dx.doi.org/10.1016/j.biortech.2023.128750 | DOI Listing |
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