Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Sperm concentration and sperm motility are the two major causes of male infertility. Having spermatozoa in semen without motility or flagellum tail defect is a major concern needed to be investigated. The CatSper genes are the novel family of four sperm-specific Ca-permeable channels which plays an important role in sperm motility, acrosome reaction, sperm, and oocyte fusion. CatSper1, CatSper2, and CatSper3 are very well-studied genes for their role in asthenozoospermia, but the association of these genes with metabolic genes is still unstudied. Another unrevealed aspect is how ROS alter the function of CatSper genes. Among the Catsper family genes, the role of CatSper4 gene must be explored more. In this study, we have used the in silico approach to find the connection between the CatSper family gene with glycolytic genes and also the involvement of CATSPER4 protein in sperm flagellum using the STRING database. Connection of CATSPER1 protein with lipid metabolic gene is also found in Reactome database, and after that gene ontology of these genes was done by using DAVID and Enrichr databases. This analysis showed a strong interaction between CATSPER1, CATSPER2, and CATSPER3 protein with glycolytic protein (i.e., GAPDHS and PGK2), and CATSPER4 protein shows strong relation in the function of sperm flagellum. We also found a novel gene, i.e., APOB contributing to sperm motility. Gene ontology showed the role of APOB and glycolytic proteins in sperm motility. Enrichr analysis showed the association of APOB and glycolytic proteins in asthenozoospermia and CATSPER4 protein with sperm flagellum. Elsevier Pathway Collection also showed proteins involved in male infertility (i.e., GAPDHS). Therefore, we report the role of the CatSper4 gene in sperm tail function and the APOB novel gene involved in sperm motility. Understanding the molecular mechanism(s) of regulations of the CatSper family gene will help us to develop new therapeutic approaches in infertile males.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/978-3-031-12966-7_18 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Effect of Kisspeptin Fortification in Freezing Media on Post-Thaw Quality and Fertility of Buffalo Bull Semen.
Reprod Domest Anim
September 2025
Department of Teaching Veterinary Clinical Complex, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India.
The present study was undertaken to assess the effect of kisspeptin supplementation (0.0, 5.0, 10.
View Article and Find Full Text PDFEvidence against the role of toll-like receptors 7 and 8 in sex selection in mice, cattle, and humans.
iScience
September 2025
Department of Animal Science, University of Connecticut, 1390 Storrs Road, Storrs, CT 06269-4163, USA.
Recent studies suggested that treating sperm with R848, a ligand for the X-linked Toll-like receptors 7 and 8 (TLR7/8) in mice, goats, and cattle, could selectively reduce the motility of X chromosome bearing sperm (X-sperm). This reduction enables the separation of X- and Y-sperm and thereby sex selection. However, through three species and multiple methods, our results challenged prior published data.
View Article and Find Full Text PDFRequirement of Lysosomal Two-Pore Channels for Normal Fertilization and Artificial Oocyte Activation in Mice.
Front Biosci (Landmark Ed)
August 2025
Department of Integrated Applied Life Science, Integrated Graduate School of Medicine, Engineering, and Agricultural Sciences, University of Yamanashi, 400-8510 Yamanashi, Japan.
Background: Lysosomes serve not only in the degradation of cellular components but also as calcium (Ca) stores. In this study, we investigated the effects of trans-Ned19, an inhibitor of lysosomal calcium channels known to block two-pore channels (TPCs), on fertilization and oocyte activation in mice.
Methods: Pronuclear formation was assessed via Hoechst 33342 staining, cortical granule release was evaluated using agglutinin-fluorescein isothiocyanate (LCA-FITC) staining, intracellular Ca levels were monitored with Cal-520 AM, and sperm motility was analyzed using a sperm motility analysis system (SMAS).
Sequence and expression analysis of potential spermatogenesis-specific gene cognates in the Caribbean fruit fly, Anastrepha suspensa.
Insect Sci
September 2025
Department of Entomology & Nematology, University of Florida, Gainesville, Florida.
The sterile insect technique (SIT) is a highly effective biologically-based method for the suppression of many insect pest populations. SIT efficacy could be improved by methods of male sterilization that avoid the use of irradiation that can result in diminished fitness and mating competitiveness. Alternative sterilization methods include conditional disruption of genes for male fertility, or using their sperm-specific promoters to drive the expression of genes for lethal effectors.
View Article and Find Full Text PDFS100A12 correlates with inflammatory and pain symptoms in patients with Chronic Prostatitis/Chronic Pelvic Pain Syndrome.
J Pain
September 2025
Clin(i)c of Urology, Pediatric Urology and Andrology, Justus-Liebig-University Giessen, Rudolf-Buchheim-Str. 7, 35392 Giessen, Germany; Molecular Andrology, Justus-Liebig-University Giessen, Schubertstr. 81, 35392 Giessen, Germany; Hessian Centre of Reproductive Medicine, Justus-Liebig-University Gi
Chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) is the most prevalent urological condition in men under 50, characterized by persistent or recurrent pelvic and perineal pain, and significantly reduced quality of life. Reliable biomarkers for assessment and mechanistic understanding of pain remain limited. This retrospective case-control study consisting of 90 CP/CPPS patients (median age 29.
View Article and Find Full Text PDF