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Conceptual and statistical models that include conditional indirect effects (i.e., so-called "moderated mediation" models) are increasingly popular in the behavioral sciences. Although there is ample guidance in the literature for how to specify and test such models, there is scant advice regarding how to best design studies for such purposes, and this especially includes techniques for sample size planning (i.e., "power analysis"). In this paper, we discuss challenges in sample size planning for moderated mediation models and offer a tutorial for conducting Monte Carlo simulations in the specific case where one has categorical exogenous variables. Such a scenario is commonly faced when one is considering testing conditional indirect effects in experimental research, wherein the (assumed) predictor and moderator variables are manipulated factors and the (assumed) mediator and outcome variables are observed/measured variables. To support this effort, we offer example data and reproducible R code that constitutes a "toolkit" to make up for limitations in other software and aid researchers in the design of research to test moderated mediation models.
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http://dx.doi.org/10.3758/s13428-022-01996-0 | DOI Listing |
Behav Brain Res
August 2025
Department of Engineering Design, Indian Institute of Technology Madras, India. Electronic address:
Test Anxiety (TA) is known to impair the heart-brain interaction affecting both the central and autonomic nervous systems. The impairment is often assumed to be uniform, overlooking individual variability in stress response. This study explores how heart-brain dysregulation in TA may manifest conditionally, shaped by individual differences.
View Article and Find Full Text PDFJ Atten Disord
August 2025
Psychiatric University Clinics Basel; Center for Affective, Stress and Sleep Disorders, Basel, Switzerland.
Background: Individuals with cognitive disengagement syndrome (CDS) report both lower physical activity levels and more insomnia than the general population. However, reliable data on adults with CDS are missing so far. The aims of the present study were three-fold: (1) to investigate the associations between CDS and physical activity patterns among young adults, and more specifically dimensions of physical activity (walking time/week, bicycling time/week, and aerobic physical activity/week), (2) to explore, if CDS scores, physical activity patterns, and insomnia were interrelated, and (3) to explore, if physical activity was directly or indirectly associated with CDS via decreased insomnia.
View Article and Find Full Text PDFBMC Psychol
August 2025
Department of Psychology, Bielefeld University, Universitätsstraße. 25, 33615, Bielefeld, Germany.
Background: Excessive study behaviour as a precursor to academic burnout is receiving increasing attention in the research landscape. However, potential risk factors for this behaviour remain largely unconsidered. Against this background, this study, based on the self-esteem model of burnout, examines the risk-increasing influence of academic self-esteem contingency on burnout and extends the empirical research on this topic by investigating the mediating effect of excessive study behaviour.
View Article and Find Full Text PDFChem Sci
August 2025
College of Pharmaceutical Sciences, Zhejiang University 866 Yuhangtang Street Hangzhou 310058 China
Detecting enzyme activity that catalyzes subtle functional group transformations in live cells remains a major challenge. We introduce a conditional metabolic labeling strategy for enzymatic activity detection (cMLEAD), which harnesses cellular metabolic pathways to deliver indirect yet reliable activity readouts. Unlike traditional metabolic labeling approaches relying on nonspecific incorporation of tagged biomolecules, cMLEAD employs a tagged precursor whose metabolic incorporation is strictly dependent on specific enzymatic activity, effectively transforming a metabolic labeling event into an enzyme-activity measurement.
View Article and Find Full Text PDFInt J Obes (Lond)
August 2025
Department of Epidemiology and Biostatistics, MRC Centre for Environment Health, School of Public Health, Imperial College, London, UK.
Objective And Methods: We have developed a novel Bayesian Linear Structural Equations Model (BLSEM) with variable selection priors (available as an R package) to build directed acyclic graphs to delineate complex variable associations and pathways to BMI development. Conditional on standard assumptions used in causal inference, the model provides interpretable estimates with uncertainty for natural direct, indirect (mediated) and total effects.
Results: We showcase our method using data on 4119 offspring followed from the pre-pregnancy period to age 46 years (y) in a Finnish population-based birth cohort.