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Article Abstract
Background: To date, few studies have investigated the feasibility of the loop-mediated isothermal amplification (LAMP) assay for identifying pathogens in tissue samples. This study aimed to investigate the feasibility of LAMP for the rapid detection of methicillin-susceptible or methicillin-resistant (MSSA or MRSA) in tissue samples, using a bead-beating DNA extraction method.
Methods: Twenty tissue samples infected with either MSSA (n = 10) or MRSA (n = 10) were obtained from patients who underwent orthopedic surgery for suspected musculoskeletal infection between December 2019 and September 2020. DNA was extracted from the infected tissue samples using the bead-beating method. A multiplex LAMP assay was conducted to identify MSSA and MRSA infections. To recognize the genus, , and methicillin resistance, 3 sets of 6 primers for the 16S ribosomal ribonucleic acid (rRNA) and the and genes were used, respectively. The limit of detection and sensitivity (detection rate) of the LAMP assay for diagnosing MSSA and MRSA infection were analyzed.
Results: The LAMP result was positive for samples containing 10 colony-forming unit (CFU)/mL for 16S rRNA, 10 CFU/mL for , and 10 CFU/mL for . The limits of detection for 16S rRNA and were not different between MSSA and MRSA. For the 10 MSSA-positive samples, the LAMP assay showed 100% positive reactions for 16S rRNA and and a 100% negative reaction for . For the 10 MRSA-positive samples, the LAMP assay showed 100% positive reactions for 16S rRNA and but only 90% positive reactions for . The sensitivity (detection rate) of the LAMP assay for identifying MSSA and MRSA in infected tissue samples was 100% and 90%, respectively.
Conclusions: The results of this study suggest that the LAMP assay performed with tissue DNA samples can be a useful diagnostic method for the rapid detection of musculoskeletal infections caused by MSSA and MRSA.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9393274 | PMC |
| http://dx.doi.org/10.4055/cios21277 | DOI Listing |
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