Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Knowing concentrations of lipids is essential for understanding their physiological functions and discovering new disease biomarkers. However, it is highly challenging to accurately quantify lipids due to structural diversity and multiple isomeric forms of lipids. To address these critical gaps, we have developed a novel aziridine-based isobaric tag labelling strategy that allows (i) determination of lipid double-bond positional isomers, (ii) accurate relative quantification of unsaturated lipids, and (iii) improvement of ionization efficiencies of nonpolar lipids. The power of this method is demonstrated in characterization and quantification of various categories of lipids such as fatty acids, phosphoglycerol lipids, cholesteryl esters (CE), and glycerides. 17 CE lipid isomers were identified and quantified simultaneously from Alzheimer's disease (AD) mouse serum without using lipid standards. Among them, 6 CE isomers showed significant changes in concentrations in AD serum.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/anie.202207098 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
High-Specificity and Sensitivity Imaging of Neutral Lipids Using Salt-Enhanced MALDI TIMS.
J Am Soc Mass Spectrom
September 2025
Department of Chemistry, Vanderbilt University, Nashville, Tennessee 37235, United States.
Neutral lipids are vital to various cellular processes and disease pathologies. However, their characterization by matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) remains challenging due to poor ionization efficiency and difficulties distinguishing subtle structural differences among numerous isomeric and isobaric species. In this study, we enhanced neutral lipid detection by incorporating isotonic metal-cation washes into our MALDI IMS sample preparation workflow.
View Article and Find Full Text PDFCharacterization of unsaturated fatty acids with high structural specificity by nuclear magnetic resonance spectroscopy.
Biochimie
August 2025
Ji Hua Laboratory, Foshan, 52800, Guangdong, China. Electronic address:
Comprehensive characterization of fatty acid (FA) structures has important value, as they are important components of cells and are crucial for cell structure and function. However, in view of the different structural possibilities of unsaturated FAs due to the carbon-carbon double bond (CC) location and its chemical configuration, there are still certain difficulties in characterizing unsaturated FAs with high structural specificity. In this work, we present a method based on nuclear magnetic resonance (NMR) spectroscopy for comprehensive characterization of unsaturated FAs.
View Article and Find Full Text PDFTotal synthesis and bioactivity investigation of a chiral diacylglycerol.
Sci Rep
August 2025
School of Pharmacy and Medical Technology, Putian University, Putian, 351100, China.
The total synthesis of compound 1, a chiral diglyceride metabolite present in both humans and fungi, was achieved via a seven-step route, affording the target molecule in 2.33% overall yield. The synthetic strategy involved: (1) selective protection of the terminal hydroxyl group of chiral ketal 2 with a sterically hindered benzyl group, followed by ketal deprotection to yield benzyl ether 4; (2) protection of the terminal hydroxyl group of benzyl ether 4 with a bulky silyl protecting group, and subsequent esterification of the remaining hydroxyl with erucic acid to generate ester 6; (3) removal of the silyl protecting group from ester 6, followed by esterification of the liberated hydroxyl group with pentadecanoic acid to afford ester 8; and (4) selective deprotection of the benzyl group of ester 8 to furnish compound 1.
View Article and Find Full Text PDFDevelopment of a pseudotargeted metabolomics approach for relative quantification of free fatty acid double-bond isomers in beagle plasma.
J Chromatogr B Analyt Technol Biomed Life Sci
August 2025
Key Laboratory of Drug Quality Control and Pharmacovigilance, China Pharmaceutical University, Ministry of Education, Nanjing 210009, PR China; State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, PR China. Electronic address:
Free fatty acids (FFAs) play a key role in living organisms and participate in metabolic processes, mainly as a source of energy for cells. The CC position isomer of FFAs is strongly associated with many diseases, but quantification of its double bond position isomer remains a challenge in lipid analysis. In this study, based on the requirements of the pseudotargeted metabolomics, an analytical method based on a two-step derivatization LC-MS/MS method and a multiple reaction monitoring (MRM) mode was established and fully validated in terms of linearity, precision, and stability.
View Article and Find Full Text PDFFormulation of Honokiol- and Magnolol-Loaded Nanoemulsions for Head and Neck Cancer Adjuvant Therapy: Evaluation of Radiation Sterilization Effects on Active Substance Properties.
Int J Mol Sci
August 2025
Chair and Department of Pharmaceutical Chemistry, Poznan University of Medical Sciences, Rokietnicka 3, 60-806 Poznan, Poland.
Honokiol (HON) and magnolol (MAG), structural isomers from , exhibit notable anticancer activity, particularly against head and neck squamous cell carcinoma (HNSCC). However, due to their high lipophilicity, their intravenous administration is challenging. This study aimed to develop HON- and MAG-loaded intravenous (IV) nanoemulsions using commercial lipid preparations with varying fatty acid compositions.
View Article and Find Full Text PDF