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Background: Delivery of a high-quality provisional restoration at a maxillary anterior immediate implant site enhances patient-centered outcomes and promotes development of favorable hard and soft tissue architecture. The purpose of this report is to present a protocol relying upon compatible guided surgery and laboratory systems for fabrication of a custom provisional crown prior to immediate implant surgery in the esthetic zone.
Methods And Results: A female patient, aged 33 years, presented to the Army Postgraduate Dental School, Fort Gordon, Georgia, with an unfavorable prognosis for tooth #9. The patient elected extraction with immediate implant placement. Prior to the surgery, we utilized a cone-beam computed tomography volume, stone models, implant planning software, and an implant indexing system to fabricate a custom provisional crown. Following extraction of tooth #9 and immediate implant placement, the provisional crown exhibited excellent fit and finish, requiring virtually no chairside adjustment. We noted minimal change in baseline mucosal contours throughout the healing phase.
Conclusion: The clinical/restorative protocol described in this report assured accurate three-dimensional implant positioning and permitted indirect fabrication of a high-quality custom provisional crown in advance of surgery. The laboratory workflow-which dental technicians/auxiliaries can master-has the potential to shorten surgery, enhance treatment outcomes, and increase patient satisfaction.
Key Points: Why is this case new information? This report provides a stepwise workflow guiding indirect fabrication of a custom provisional crown prior to immediate implant placement. What are the keys to successful management of this case? The described technique requires compatible laboratory and guided surgery systems to assure that the restoration accounts for the three-dimensional position and timing of the implant. What are the primary limitations to success in this case? Dental technicians/auxiliaries can master this protocol and independently produce high-quality provisional implant restorations under supervision, potentially enhancing practice efficiency. However, practitioners should provide adequate staff training to optimize reliability and quality.
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http://dx.doi.org/10.1002/cap.10215 | DOI Listing |
Respir Investig
August 2025
Department of Respiratory Medicine, Kanagawa Cardiovascular and Respiratory Center, 6-16-1 Tomiokahigashi, Kanazawa-ku, Yokohama, Kanagawa, 236-0051, Japan.
Background: Diagnosing pulmonary Mycobacterium avium complex (MAC) disease in asymptomatic patients is challenging due to difficulty in collecting sputum culture. This study evaluated the clinical and characteristics and outcomes of patients diagnosed through gastric aspirate culture to assess its usefulness.
Methods: We retrospectively analyzed 125 patients with radiological findings consistent with pulmonary MAC disease who had positive MAC cultures from gastric aspirate between April 2014 and March 2022.
Nat Commun
August 2025
Department of Electrical and Computer Engineering, The University of British Columbia, Vancouver, BC, Canada.
The scanning electron microscope (SEM) delivers high resolution, high depth of field, and an image quality as if microscopic objects are seen by the naked eye. This makes it not only a powerful scientific instrument, but a tool inherently applicable to nearly all fields of study and curiosity involving the small scale. However, SEMs have remained complex, expensive, and beyond the reach of many.
View Article and Find Full Text PDFStem Cell Reports
August 2025
Neural Stem Cell Institute, 150 New Scotland Ave., Albany, NY 12208, USA.
The retinal pigment epithelium (RPE) is a pigmented monolayer of cells beneath the neural retina that supports photoreceptor cell function essential for vision. Our study explores the diversity of adult human RPE subpopulations and associated implications for retinal biology. Employing cellular indexing of transcriptomes and epitopes by sequencing (CITE-seq), we identified distinct RPE cell subpopulations characterized by unique single-cell transcriptomic and surface protein signatures.
View Article and Find Full Text PDFBioengineering (Basel)
July 2025
DOE Joint BioEnergy Institute, Emeryville, CA 94608, USA.
Electroporation-mediated gene delivery is a cornerstone of synthetic biology, offering several advantages over other methods: higher efficiencies, broader applicability, and simpler sample preparation. Yet, electroporation protocols are often challenging to integrate into highly multiplexed workflows, owing to limitations in their scalability and tunability. These challenges ultimately increase the time and cost per transformation.
View Article and Find Full Text PDFAutophagy Rep
August 2025
Lysosomal Health in Ageing, Lifelong Health, South Australian Health and Medical Research Institute, Adelaide, SA, Australia.
Macroautophagy (referred to here as autophagy) is thought to play a critical role in aging and age-related disease, making it a priority for development of targeted human therapies. We developed a flow cytometry-based method to measure autophagic flux in 19 subpopulations from whole blood, using chloroquine (CQ) to inhibit lysosomal degradation, and the autophagy protein MAP1LC3B (microtubule associated protein 1 light chain 3 beta) isoform II/LC3B-II to measure autophagic flux (the acquisition and degradation of autophagic cargo over time). Autophagic flux varies by cell type and is higher in whole blood compared with RPMI culture media.
View Article and Find Full Text PDF