The Relation Between Host TLR9 -1486T/C, rs187084 Gene Polymorphisms and , , , and Virulence Genes among Gastric Cancer Patients.

To identify the associations between different genotypes of TLR9 -1486T/C (rs187084) with gastric cancer patients and reveal their relation to virulence genes (, , and ). Patients with gastric cancer were recruited to our study, diagnosed both endoscopically and histopathologically. were isolated from gastric samples by culture and PCR amplification of the gene. Virulence genes , , , and were detected by multiplex PCR. Blood samples were used for genotyping of TLR9 -1486T/C (rs187084) by PCR-RFLP. Out of 132 patients with gastric cancer, 106 (80.3%) were positive for . A similar number of healthy participants was recruited as controls. The prevalence of , and genes among was 90.6%, 70.8%, 83.0%, and 95.3%, respectively. The gene alleles had a prevalence of 95.3% for , 52.8% for , and 42.5% for . The CC genotype of TLR9 -1486T/C had a significantly higher frequency in gastric cancer patients when compared to healthy participants ( = 0.045). Furthermore, the CC genotype demonstrated a significant association with strains carrying , , and virulence genes ( = 0.021, = 0.049, and = 0.048 respectively). Patients with CC genotype of TLR9 -1486T/C (rs187084) might be at higher risk for the development of gastric cancer, and its co-existence with strains carrying , , or virulence genes might have a synergistic effect in the development of gastric cancer. Further studies on a wider scale are recommended.