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The family Buthidae represents an early-diverging and most species-rich lineage of extant scorpions, but its internal phylogenetic relationships are still poorly understood. The family is traditionally divided into six morpho-groups; however, the monophyly of some of them remains unclear. We combined multilocus sequence data with extensive taxon sampling to reconstruct the phylogenetic relationships among Buthidae and assess the validity of the morphology-based groupings. We recovered a monophyletic Buthus group as a sister clade to all the remaining Buthidae. We also found support for the monophyly of the Tityus group, but the remaining morpho-groups were recovered as para-/polyphyletic. Our results also suggest that some genera are in need of a taxonomic revision.
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http://dx.doi.org/10.1016/j.ympev.2022.107511 | DOI Listing |
Mol Pharmacol
August 2025
Division of Preclinical Innovation, National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, Maryland. Electronic address:
Although multiparameter cellular morphological profiling methods and three-dimensional (3D) biological model systems can potentially provide complex insights for pharmaceutical discovery campaigns, there have been relatively few reports combining these experimental approaches. In this study, we used the U87 glioblastoma cell line grown in a 3D spheroid format to validate a multiparameter cellular morphological profiling screening method. The steps of this approach include 3D spheroid treatment, cell staining, fully automated digital image acquisition, image segmentation, numerical feature extraction, and multiple machine learning approaches for cellular profiling.
View Article and Find Full Text PDFMar Environ Res
October 2025
Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research, Am Handelshafen 12, 27570 Bremerhaven, Germany; Marine Zoologie, Fachbereich 2, University of Bremen, Bremen, Germany.
The marine ecosystem of Kongsfjorden (KF), Svalbard, is directly affected by ongoing climate change in the Arctic. Shifts in species composition and distributions are already underway as a result of the transition from Arctic to Atlantic conditions in the fjord. The polar night is a period of challenging conditions and is historically understudied, and thus our understanding of the biodiversity of major eukaryotic groups is particularly limited at this time of year.
View Article and Find Full Text PDFProc Biol Sci
August 2025
Naturhistorisches Museum Basel, Basel 4051, Switzerland.
Currently, little is known about the early evolution and geographical origin of Salamandridae, the most species-rich family of Palearctic salamanders. The description of new fossil material from the early Palaeogene of the Paris Basin provides new insight into the history of amphibians and for the first time allows the inclusion of the oldest salamandrid taxa into a phylogenetic framework, based on a new morphology-based character matrix. By comparing the evolutionary trajectories of the two main Nearctic groups of Cenozoic amphibians, urodeles and anurans, it emerges that whereas frogs were hardly affected by the Palaeocene-Eocene Thermal Maximum (PETM), salamanders responded in Western Europe with a diversification event, corresponding to a spread of metamorphosing, likely terrestrial, salamandrids.
View Article and Find Full Text PDFJ Clin Med
July 2025
King's Fertility, Fetal Medicine Research Institute, London SE5 8BB, UK.
: Embryo selection in IVF is traditionally based on morphology, yet many high-quality embryos fail to implant. Preimplantation genetic testing for aneuploidy (PGT-A) using next-generation sequencing (NGS) has been proposed to improve selection by identifying euploid embryos. However, its effectiveness in women of advanced maternal age remains unclear due to limited randomized data.
View Article and Find Full Text PDFJ Vis Exp
July 2025
Ateneo Biodiversity Research Laboratory, Department of Biology, School of Science and Engineering, Higher Education Cluster, Ateneo de Manila University; Ateneo School of Medicine and Public Health, Higher Education Cluster, Ateneo de Manila University.
Biodiversity decline is transpiring at rates unimaginable, yet biodiversity monitoring is hampered by a plethora of factors, including the incomplete inventory of baseline diversity and the dwindling population of skilled taxonomists. Bridging this impediment in species inventory of "dark taxa", or hyperdiverse groups often understudied in taxonomy, is particularly crucial in the tropics facing insurmountable environmental pressures. While morphology-based alpha-taxonomy remains the gold standard in species identification, DNA-based methods have been showing tremendous potential in accelerating species delineation and discovery.
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