A Concoction Pipeline for Generating Molecular Operational Taxonomic Units (MOTUs) Among Riparian and Aquatic Beetles.

Biodiversity decline is transpiring at rates unimaginable, yet biodiversity monitoring is hampered by a plethora of factors, including the incomplete inventory of baseline diversity and the dwindling population of skilled taxonomists. Bridging this impediment in species inventory of "dark taxa", or hyperdiverse groups often understudied in taxonomy, is particularly crucial in the tropics facing insurmountable environmental pressures. While morphology-based alpha-taxonomy remains the gold standard in species identification, DNA-based methods have been showing tremendous potential in accelerating species delineation and discovery.

Two new species of Thomson, 1859 (Coleoptera, Hydrophilidae) from Northern Luzon, Philippines.

Two species of Thomson, 1859, and , are described from Northern Luzon, Philippines. The new species can be distinguished through colour, body shape, surface puncturation and characteristic aedeagi. Descriptions are provided and complemented with habitus photographs and drawings of the aedeagi.

Two new species of Motschulsky, 1858 (Coleoptera, Limnichidae, Limnichinae) from Negros, Philippines.

Two new species of Limnichidae beetles, and , are described from the Island of Negros in the Philippines. The adult specimens of the new species can be differentiated by patterns of body punctation, colour and orientation of elytral pubescence, posterolateral angle of pronotum, tarsomere length ratio and aedeagal form. Two clades, representing the two new species, were retrieved in the Maximum Likelihood gene tree using the 3'-end of the gene.

(s.str.) , new species (Coleoptera: Hydraenidae) along with further records of spp. from Durmitor National Park, Montenegro and comments on the DNA barcoding problem with the genus.

Background: Long-palped Water Beetles were collected during a taxon expedition in Montenegro which involved citizen scientists, students and taxonomists. The material was collected from springs, brooks, fens and the Tara River, at altitudes between 600 m and 1450 m above sea level, using fine-meshed hand-nets and by manual checking of submerged substrates. The morphological species delimitation was supplemented and congruent with mtDNA sequences mainly obtained in the field using the newly-developed MinION-based pipeline.