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Article Abstract
Microdroplet emulsions allow investigators to build controllable microenvironments for applications in experimental evolution and synthetic ecology. We designed a microfluidic platform that uses highly homogenous microdroplets to enable these experiments. We also present a step-by-step protocol for the rapid production of highly homogeneous microdroplets suitable for experimental evolution. We also describe protocols for the propagation and serial passage of microbial populations across a range of selection schemes and potential spatial structures. For complete details on the use and execution of this protocol, please refer to Seo et al. (2021).
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9048157 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2022.101332 | DOI Listing |
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