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Article Abstract
Blood endothelial cells (BECs) in lymph nodes are distinct stromal cells with a transcriptional profile allowing fast and specific adaptation to the functional requirements. Here, we describe a step-by-step protocol for the enzymatic digestion of lymph nodes, the enrichment of stromal cells, the sorting of BECs, and the processing of BEC-related data for modern analysis approaches as spectral flow cytometry and single-cell RNA sequencing (scRNA-seq). For complete details on the use and execution of this protocol, please refer to Menzel et al. (2021).
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8991252 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2022.101267 | DOI Listing |
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