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Article Abstract
CGA009 is a metabolically robust microbe that can utilize lignin breakdown products to produce polyhydroxyalkanoates (PHAs), biopolymers with the potential to replace conventional plastics. Our recent efforts suggest PHA granule formation is a limiting factor for maximum production of the bioplastic poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) by The Phap1 phasin () from the PHB-producing model bacterium H16 was expressed in with the aim of overproducing PHBV from the lignin breakdown product coumarate by fostering smaller and more abundant granules. Expression of yielded PHBV production from aerobically (0.7 g/L), which does not occur in the wild-type strain, and led to a significantly higher PHBV titer than wild-type anaerobic production (0.41 g/L). The 3HV fractions were also significantly increased under both anaerobic and aerobic conditions, which boosts thermomechanical properties and potential for application. Thus, heterologous phasin expression in provides flexibility for industrial processing and could foster compositional changes in copolymers with better thermomechanical properties compared to PHB alone.
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| http://dx.doi.org/10.1016/j.mec.2021.e00191 | DOI Listing |
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