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Marine phages have been applied to trace ground- and surface water flows. Yet, information on their transport in soil and related particle intactness is limited. Here we compared the breakthrough of two lytic marine tracer phages (Pseudoalteromonas phages PSA-HM1 and PSA-HS2) with the commonly used Escherichia virus T4 in soil- and sand-filled laboratory percolation columns. All three phages showed high mass recoveries in the effluents and a higher transport velocity than non-reactive tracer Br. Comparison of effluent gene copy numbers (CN) to physically-determined phage particle counts or infectious phage counts showed that PSA-HM1 and PSA-HS2 retained high phage particle intactness (I > 81%), in contrast to T4 (I < 36%). Our data suggest that marine phages may be applied in soil to mimic the transport of (bio-) colloids or anthropogenic nanoparticles of similar traits. Quantitative PCR (qPCR) thereby allows for highly sensitive quantification and thus for the detection of even highly diluted marine tracer phages in environmental samples.
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http://dx.doi.org/10.1016/j.scitotenv.2021.152704 | DOI Listing |
Environ Sci Technol
September 2025
IPREM, Institut des Sciences Analytiques et de Physico-chimie pour l'Environnement et les Matériaux, E2S-UPPA, CNRS, Université de Pau et des Pays de l'Adour, 2 Av. du Président Pierre Angot, Pau 64000, France.
The origin of the bioaccumulative neurotoxin methylmercury (MeHg) in the ocean remains elusive. The current paradigm suggests that the microbial methylation of inorganic Hg within the oceanic water column produces monomethylmercury (MMHg) and potentially dimethylmercury (DMHg). Reaction rates and main drivers governing MeHg levels (sum of MMHg and DMHg) are poorly constrained.
View Article and Find Full Text PDFToxics
July 2025
Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China.
Cadmium (Cd) pollution poses a serious threat to freshwater ecosystems. The freshwater mussel is increasingly used as a bioindicator for monitoring Cd pollution in aquatic environments. However, the primary routes of Cd accumulation in remain unclear, and the molecular regulatory mechanisms underlying Cd accumulation are poorly understood.
View Article and Find Full Text PDFAppl Radiat Isot
August 2025
Nuclear Engineering Laboratory, National Technical University of Athens, 15780 Athens, Greece.
Radon short-lived progeny may be used as tracers of environmental processes. Monitoring their activity in ambient air is a challenging process, and full interpretation of their fluctuations remains unclear. In this work, an enhanced technique for monitoring radon progeny in ambient air is presented.
View Article and Find Full Text PDFJ Agric Food Chem
August 2025
Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan.
metabolism of epoxy fatty acids, the unsaturated fatty acid oxidation products formed upon heating of edible oils, remains unclear. Here, we compared the catabolic rates and accumulation of dietary and epoxy octadecanoic acids (epoxy-C18:0) with those of oleic acid (C18:1) in mice using stable isotope-labeled compounds. Both epoxy isomers were catabolized more rapidly than C18:1, with epoxy-C18:0 exhibiting a slightly faster rate than epoxy-C18:0.
View Article and Find Full Text PDFEnviron Res
August 2025
Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems and Fujian Provincial Key Laboratory for Coastal Ecology and Environmental, College of the Environment and Ecology, Xiamen University, Fujian, 361102, China.
Under the global HFCs reduction framework of the Kigali Amendment (KA) to the Montreal Protocol (MP), although HFC-152a is widely used as an ozone-friendly alternative in fields such as refrigeration and foam blowing agents, its production volume has gradually increased in recent years, prompting regulatory attention. In this study, we conducted a continuous daily in-situ observation of HFC-152a at a remote site in southeastern China from May 2023 to May 2024. HFC-152a exhibits seasonal variations in mixing ratios and enhancements, with summer values being lower than in other seasons.
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