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In this study, a novel pretreatment of cation exchange resin (CER) coupled NaCl addition was proposed to enhance waste activated sludge (WAS) hydrolysis and promote short-chain fatty acids (SCFAs) production in the anaerobic fermentation process. At the optimal pretreatment condition of 3 g/g SS CER and 15 g/L NaCl, considerable SCOD (i.e. 5107 mg/L, 35.4% of TCOD) was released after 2-day coupled treatment, which provided sufficient organic substance for the subsequent SCFAs production. The sludge hydrolysis mechanism was illustrated, i.e. CER triggered extracellular polymeric substances (EPS) disruption and NaCl induced microbial cells lysis. The synergistic interaction between CER and NaCl pretreatment was investigated and application potential of fermentative liquid was evaluated after the coupled pretreatment-enhanced anaerobic fermentation. In the presence of abundant biodegradable substrates in the fermentative liquid, 4742 mg COD/L (i.e. 388 mg COD/g VSS) of SCFAs production was achieved within 6-day anaerobic fermentation, mainly composed of acetic and propionic acids (70.4% of total SCFAs).
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http://dx.doi.org/10.1016/j.jenvman.2021.114074 | DOI Listing |
J Agric Food Chem
September 2025
Department of Chemistry and Chemical Engineering, Engineering Research Center of Forestry Biomass Materials and Bioenergy (Ministry of Education), National Forest and Grass Administration Woody Spices (East China) Engineering Technology Research Center, Beijing Forestry University, Beijing 100083, C
This study develops a catalytic system using pyruvic acid (PYA) and Fe to efficiently coproduce xylo-oligosaccharides (XOS) and (manno-oligosaccharides) MOS from food material ( Lam. fruit.) and its waste peel, respectively.
View Article and Find Full Text PDFInfect Disord Drug Targets
August 2025
Department of Biochemistry and Microbiology, North South University, Bashundhara, Dhaka, Bangladesh.
Mitochondria are the cellular powerhouses and are considered to be central to energy metabolism, dynamics, and homeostasis. There is growing evidence that the gut microbiome regulates mitochondrial biogenesis, dynamics (fission, fusion, mitoph-agy), and bioenergetics, and that it does so by connecting bacterial metabolites and signaling molecules. This review discusses the molecular mechanisms that underlie the interplay between bacteria and mitochondria, with a particular focus on the modulation of mitochondrial activities by microbial products, including bile acids, immunological mediators, and short-chain fatty acids (SCFAs).
View Article and Find Full Text PDFFood Funct
September 2025
Department of Nutrition, University of California, Davis, Davis, California, USA.
To determine the fermentation capacity of gut microbiomes with diverse plant carbohydrate active enzyme (CAZyme) repertoires, we collected fecal samples from 18 healthy adults who reported consuming at least 5 different fruits and vegetables daily and conducted shotgun metagenome analysis. Five fecal samples with the most diverse CAZymes were then fermented with 7 different fibers selected for their unique monosaccharide profiles-banana, kale,13-bean soup, flax, coconut flour, MS Prebiotic (resistant starch) and Sunfiber (guar gum)-for 72 hours. Samples were collected at 4 timepoints for 16S sequencing, and pH, SCFAs, and monosaccharide measurements.
View Article and Find Full Text PDFInt J Biol Macromol
September 2025
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China; State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, 214122, China. Electronic address:
This study investigated fermentation-induced alterations in the structural and physicochemical properties of Brassica rapa L. polysaccharide (BRL-G) and their effects on gut microbiota composition. An in vitro continuous bioreactor system was used, combining ultrasound-microwave-assisted enzymatic extraction with fecal microbiota co-culture.
View Article and Find Full Text PDFJ Nutr
September 2025
Institute of Food and One Health, Leibniz University Hannover, 30167 Hannover, Germany.
Background: Dietary fiber supports metabolic health via microbial fermentation, producing short-chain fatty acids (SCFAs). However, metabolic responses to fiber vary between individuals, potentially due to differences in gut microbiota composition. The Prevotella-to-Bacteroides (P/B) ratio has emerged as a potential biomarker for fiber responsiveness.
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