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Article Abstract
This protocol describes how to order and directly assemble uracil-containing non-clonal DNA fragments by uracil excision based cloning (USER cloning). The protocol was generated with the goal of making synthesized non-clonal DNA fragments directly compatible with USER cloning. The protocol is highly efficient and would be compatible with uracil-containing non-clonal DNA fragments obtained from any synthesizing company. The protocol drastically reduces time and handling between receiving the synthesized DNA fragments and transforming with vector and DNA fragment(s).
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8438380 | PMC |
| http://dx.doi.org/10.21769/BioProtoc.2615 | DOI Listing |
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