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Methane (CH), carbon dioxide (CO), and oxygen (O) are the major gases produced by dairy cattle as a result of rumen fermentation and metabolism, and thus, their concentrations are frequently measured as a way of estimating heat production and energy metabolism. A well-utilized method of measuring gas consumption and production to estimate heat production is indirect calorimetry, which requires bags to retain the sampled gases until analysis. The objective of this study was to determine the ability of a polyvinyl fluoride gas bag (PF) and a multilayer fabrication gas bag containing an aluminum layer (NAP) to maintain respiratory gas composition in comparison to a polyethylene terephthalate bag (PET).
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