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To promote efficiency nitrogen-rich wastewater treatment from a sequencing batch biofilm reactor (SBBR), three aerobic denitrifiers (Pseudomonas mendocinaIHB602, Methylobacterium gregansDC-1 and Pseudomonas stutzeriIHB618) with dual-capacities of strong auto-aggregation and high nitrogen removal efficiency were studied. The aggregation index analysis indicated that coaggregation of the three strains co-existed was better when compared with one or two strains grown alone. Optimal coaggregation strains were used to bioaugmente a test reactor (SBBR), which exhibited a shorter time for biofilm-formation than uninoculated control reactor (SBBR). With different influent ammonia-N loads (150, 200 and 300 mg·L), the average ammonia-N and nitrate-N removal efficiency were all higher than that in SBBR, as well as a lower nitrite-N accumulation. Microbial community structure analysis revealed coaggregation strains may successfully colonize in the bioreactor and be very tolerant of high nitrogen concentrations, and contribute to the high efficiency of inorganic nitrogen-removal and biofilm-formation.
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http://dx.doi.org/10.1016/j.biortech.2021.125391 | DOI Listing |
Bioresour Technol
August 2025
Green Technologies Research Group, Facultad de Ingeniería y Ciencias Aplicadas, Universidad de los Andes, Chile. Electronic address:
The integration of sequential nitrification/mixotrophic denitrification (SNMD) in a single bioreactor is rarely reported. This study adapted an industrial inoculum to SNMD in a single bioreactor configuration, comparing a conventional sequencing batch reactor (SBR) and a zeolite-carrier SBR (SBBR) over 276 days across three phases: sequential nitrification heterotrophic denitrification (SNHD) adaptation, increased TAN loading, and SNMD with sulfide (HS-S) addition. Under SNHD conditions, both systems achieved > 99 % TAN removal with complete NO-N reduction.
View Article and Find Full Text PDFEnviron Res
July 2025
Key Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510380, China; Guangdong Ecological Remediation of Aquaculture Pollution Research Center, Guangzhou, 510380, China.
During the centralized discharge of winter aquaculture wastewater, low temperature (≤15 °C) and low C/N ratio (≤5) result in delayed initiation of the biofilm process and decreased nitrogen removal efficiency.The quorum sensing (QS) communication system serves as the core mechanism regulating biofilm formation. This study examined how adding N-butyyl-L-homo-serine lactone (C4-HSL) affects the start-up and nitrogen removal in sequential batch biofilm reactors (SBBR) operated at 14 °C with a C/N ≤ 5.
View Article and Find Full Text PDFBioresour Technol
November 2025
Department of Civil & Environmental Engineering, University of South Florida, United States. Electronic address:
Conventional biological nitrogen removal (BNR) processes for mainstream municipal wastewater (MMW) treatment have high energy and chemical costs. Partial nitritation/anammox (PN/A) has the potential to reduce the carbon footprint of BNR; however, its implementation for MMW treatment has been limited by the low ammonium and high organic matter concentrations in MMW, which prevent suppression nitrite oxidizing bacteria (NOB) and heterotrophic denitrifiers. In this study, after organic carbon diversion, ammonium was separated from MMW in a novel bench-scale sequencing batch biofilm reactor (SBBR) containing chabazite, a natural zeolite mineral with a high ammonium ion exchange (IX) capacity.
View Article and Find Full Text PDFBioresour Technol
November 2025
State Key Laboratory Breeding Base of Marine Genetic Resources, Third Institute of Oceanography, MNR, Xiamen, PR China. Electronic address:
Sulfur autotrophic denitrification (SADN) is a promising nitrogen removal process for industrial wastewater treatment, but elevated salinity is a common inhibitory factor that affects SADN performance. In this study, the sequencing batch biofilm reactor (SBBR) was enhanced with isolated salt-tolerant Sulfurimonas strains. Results showed that bioaugmentation improved the reactor's denitrification efficiency from 70.
View Article and Find Full Text PDFJ Environ Manage
July 2025
CAS Key Laboratory of Urban Pollutant Conversion, Department of Environmental Science and Engineering, University of Science and Technology of China, Hefei, 230026, PR China.
Biofilm-based technologies, particularly the sequencing biofilm batch reactor (SBBR), have emerged as a robust solution for high-salinity wastewater treatment. However, there were contradiction in the performance on high-salinity wastewater treatment of suspended carrier and fibrous carrier in SBBR. Additionally, it was seldom that biofilm formation, recovery capability, and pollutant removal for different carriers were systematically studied during the gradually increasing salinity condition.
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