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Article Abstract
Objective: To investigate the regulatory effect of zyxin on the distribution of platelet cytoskeleton.
Methods: Platelets were isolated from zyxin-knockout (Zyx) and wild type (WT) mice respectively and corresponding platelet cytoskeleton components were separated. The expressions of β-actin, α-actinin, filamin A and myosin ⅡA in cytoskeleton components were detected by Western blot. Actin polymerization was induced by the actin polymerization inducer Jasplakinolide (Jas) in WT and Zyx platelets. Also, the expressions of the cytoskeleton proteins in cytoskeleton components were detected by Western blot. WT and Zyx platelets were allowed to spread on fibrinogen-coated surface. Platelet F-actin was labeled with Alexa Fluor 488-conjugated phalloidin and the fluorescent intensity was compared between WT and Zyx platelets.
Results: After zyxin gene was knockout, the expressions of cytoskeleton proteins β-actin, α-actinin, filamin A, and myosin Ⅱ A in resting and Jas-induced platelets were significantly increased. In the platelet spreading on fibrinogen surface, F-actin was increased in Zyx platelets as compared with that in WT platelets.
Conclusion: Zyxin significantly regulates the distribution of platelet cytoskeleton, which plays an important role in maintaining platelet cytoskeleton homeostasis.
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| http://dx.doi.org/10.19746/j.cnki.issn.1009-2137.2021.03.035 | DOI Listing |
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