Interferes with Streptococci Spatial Distribution and with Protein Expression of Species within a Polymicrobial Oral Biofilm.

We asked whether transient in the oral environment synergistically interacts with orally associated bacterial species such as , , , , , and (six-species control biofilm 6S). For this purpose, four modified biofilms with seven species that contain either the wild type strain of the genotype (USA300-MRSA WT), its isogenic mutant with MSCRAMM deficiency (USA300-MRSA ΔMSCRAMM), a methicillin-sensitive (ST72-MSSA-) or a methicillin-resistant (USA800-MRSA) grown on hydroxyapatite disks were examined. Culture analyses, confocal-laser-scanning microscopy and proteome analyses were performed. strains affected the amount of supragingival biofilm-associated species differently. The deletion of MSCRAMM genes disrupted the growth of and the distribution of and within the biofilms. In addition, caused shifts in the number of detectable proteins of other species in the 6S biofilm. (USA300-MRSA WT), aggregated together with early colonizers such as and streptococci, influenced the number of secondary colonizers such as and was involved in structuring the biofilm architecture that triggered the change from a homeostatic biofilm to a dysbiotic biofilm to the development of oral diseases.