Transcriptome and gene expression analysis of (Coleoptera: Curculionidae) during developmental stages.

PeerJ

Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture China, Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, China.

Published: November 2020


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Article Abstract

Background: Red palm weevil, Olivier, is one of the most destructive pests harming palm trees. However, genomic resources for are still lacking, limiting the ability to discover molecular and genetic means of pest control.

Methods: In this study, PacBio Iso-Seq and Illumina RNA-seq were used to generate transcriptome from three developmental stages of (pupa, 7th-instar larva, adult) to increase the understanding of the life cycle and molecular characteristics of the pest.

Results: Sequencing generated 625,983,256 clean reads, from which 63,801 full-length transcripts were assembled with N50 of 3,547 bp. Expression analyses revealed 8,583 differentially expressed genes (DEGs). Moreover, gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed that these DEGs were mainly related to the peroxisome pathway which associated with metabolic pathways, material transportation and organ tissue formation. In summary, this work provides a valuable basis for further research on the growth and development, gene expression and gene prediction, and pest control of .

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7643551PMC
http://dx.doi.org/10.7717/peerj.10223DOI Listing

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