A complete set of monosomic alien addition lines developed from in a background: genotypic and phenotypic characterization.

(BB) is a valuable wild resource for the genetic improvement of (AADD) in terms of fiber quality and disease and pest resistance, but the inherent difficulties in distant hybridization hinder its utilization in breeding programs. Monosomic alien addition lines (MAALs) are powerful tools for interspecific gene transfer. First, to access useful genes from , a fertile hexaploid from × was obtained and then additional chromosomes were selected using SSR markers in successive backcrosses and self-crossing from BCF to BCF. Finally, a complete set of 13 MAALs were developed. All the MAALs were confirmed by chromosome-specific anchored SSRs and genome-wide resequencing. The MAALs demonstrated abundant variation in morphological, agronomic, yield-related, and fiber quality traits. MAAL_3B had excellent fiber strength and fineness, indicating that the transmitted chromosome may carry desirable genes for the observed phenotypes. This complete set of MAALs will provide important genetic bridge material for the identification and introgression of favorable genes from and lay an important foundation for the genetic improvement of cotton.