Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Background: The pathogenesis of arterial and venous thrombosis is in large part interlaced. How much platelet phenotype relates to acute venous thromboembolism (VTE) independent of the underlying cardiovascular profile is presently poorly investigated.
Methods: Platelet count and mean platelet volume (MPV), platelet aggregation in whole blood and platelet rich plasma (PRP), platelet-dependent thrombin generation (TG) and platelet surface activation markers were measured under standardized conditions. Machine learning was applied to identify the most relevant characteristics associated with VTE from a large array (N = 58) of clinical and platelet-related variables.
Findings: VTE cases (N = 159) presented with lower platelet count and MPV vs controls (N = 140). Whole blood aggregation showed shorter collagen/Epinephrine closure times in cases, particularly within acetylsalicylic acid (ASA) users. Within ASA users, higher PRP aggregation after adenosine diphosphate (ADP), epinephrine, collagen and arachidonic acid was observed in cases vs controls. Within non-ASA and/or subjects on anticoagulants, cases presented with lower aggregation after ADP and collagen vs controls. Lower platelet-dependent TG, higher CD63 on resting and lower PAC-1 expression after collagen/ADP in-vitro stimulated platelets further characterized VTE cases vs controls, independent of therapy. Lasso regression analysis identified 26 variables associated with VTE of which 69% were platelet-related.
Interpretation: Comprehensive phenotyping of platelet function identified a large proportion of low responders to ASA in VTE cases. Lower platelet-dependent TG and lower platelet reactivity after ex-vivo stimulation characterized the "platelet exhausted syndrome" in cases. Finally, from a large array of covariates including clinical risk factors, platelet biomarkers comprised 69% of all selected variables differentiating VTE cases vs controls.
Funding: German Federal Ministry of Education and Research, CTH-Mainz and Bayer AG.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7494681 | PMC |
| http://dx.doi.org/10.1016/j.ebiom.2020.102978 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Post-operative Venous Thromboembolism Prophylaxis for Non-arthroplasty Knee Surgery: A Closed Loop Clinical Audit.
Cureus
August 2025
Spinal Surgery, Royal National Orthopaedic Hospital, London, GBR.
Background: Venous thromboembolism (VTE) is a preventable complication following orthopaedic surgery. While most guidelines focus on arthroplasty, a significant number of knee surgeries fall under non-arthroplasty procedures, where post-operative VTE prophylaxis recommendations vary depending on anaesthetic time and weight-bearing status. National guidelines and available literature suggest the use of VTE prophylaxis for these cases, yet adherence in clinical practice remains inconsistent.
View Article and Find Full Text PDFImmunomodulation and Thrombolytic Approaches in the Management of Deep Vein Thrombosis and Pulmonary Embolism.
Cardiol Cardiovasc Med
August 2025
Department of Translational Research, College of Osteopathic Medicine of the Pacific, Western University of Health Sciences, Pomona, California 91766 USA.
Deep vein thrombosis (DVT) and pulmonary embolism (PE) are key initiating events in the development of venous thromboembolism (VTE), a condition associated with significant morbidity, mortality, and long-term complications. While traditional therapies have focused on anticoagulation and thrombolysis, current evidence describes the pivotal role of immune pathways in the pathogenesis and progression of thrombosis. This review explores the multifaceted mechanisms underlying DVT and PE, emphasizing the contribution of inflammation, leukocyte activation, and immuno-thrombosis to thrombus formation and embolization.
View Article and Find Full Text PDFA 10-year review of iliofemoral deep vein thrombosis - are they more dangerous than their distal counterparts?
J Thromb Thrombolysis
September 2025
Department of Haematology, Northern Hospital, 185 Cooper St, Epping, VIC, 3076, Australia.
Iliofemoral deep vein thrombosis (IFDVT) is associated with potential for poor outcomes despite optimal anticoagulation therapy. To characterize the real-world management of IFDVT in an Australian population. Retrospective evaluation of IFDVT cases managed at Northern Health, Australia from January 2011 to December 2020 was performed and compared to non-iliofemoral lower limb DVTs (non-IFDVT) (n = 1793).
View Article and Find Full Text PDFPhysical activity before venous thromboembolism and risk of recurrence in a population-based inception cohort.
J Thromb Thrombolysis
September 2025
Department of Epidemiology, University of Washington, 1410 NE Campus Pkwy, Seattle, WA, 98195, USA.
Introduction: The association between moderate-to-vigorous physical activity (MVPA) and recurrent venous thromboembolism (VTE) is unclear, but an improved understanding could inform behavioral health recommendations.
Methods: The Heart and Vascular Health study, set in a large integrated healthcare system, identified adults with a validated incident VTE between January 2002 and December 2010. An inception cohort was formed from these cases and followed for a first recurrent VTE through December 2014.
The Safety of Patients Who Have Preoperative Venous Thromboembolism Undergoing Joint Arthroplasty.
J Arthroplasty
September 2025
Division of Sports Medicine and Adult Reconstructive Surgery, Department of Orthopedic Surgery, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, 321 Zhongshan Road, Nanjing 210008, Jiangsu, PR China; State Key Laboratory of Pharmaceutical Biotechnology, Nanjing
Background: Some patients requiring joint arthroplasty are predisposed to preoperative venous thromboembolism (VTE). However, the impact of preoperative VTE has been infrequently reported. The objective was to investigate the risk factors and postoperative changes of preoperative VTE and assess joint arthroplasty safety.
View Article and Find Full Text PDF