Article Synopsis
- Measuring thermogenesis in individual cells is challenging due to complex biochemical environments and unclear heat transfer mechanisms.
- A novel fluorescence microscope system allows for precise intracellular temperature mapping with an accuracy of 0.2 K using luminescent probes in breast cancer cells.
- The technique reveals significant temperature variations within the cells, showing differences up to 20 degrees and gradients of about 5 degrees, which can help study thermogenic activity in organelles and intracellular processes.
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Article Abstract
Measurement of thermogenesis in individual cells is a remarkable challenge due to the complexity of the biochemical environment (such as pH and ionic strength) and to the rapid and yet not well-understood heat transfer mechanisms throughout the cell. Here, we present a unique system for intracellular temperature mapping in a fluorescence microscope (uncertainty of 0.2 K) using rationally designed luminescent Ln-bearing polymeric micellar probes (Ln = Sm, Eu) incubated in breast cancer MDA-MB468 cells. Two-dimensional (2D) thermal images recorded increasing the temperature of the cells culture medium between 296 and 304 K shows inhomogeneous intracellular temperature progressions up to ∼20 degrees and subcellular gradients of ∼5 degrees between the nucleolus and the rest of the cell, illustrating the thermogenic activity of the different organelles and highlighting the potential of this tool to study intracellular processes.
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| http://dx.doi.org/10.1021/acs.nanolett.0c02163 | DOI Listing |
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