Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Objective: Identification of specific bacteria in root canals (RCs) in distinct clinical conditions can support the comprehension of pathological processes. Thus, the objective of this clinical study was to investigate the presence of F. alocis in RCs of teeth with primary endodontic infection (PEI) and with persistent/secondary endodontic infection (SEI) by using molecular techniques. It was also aimed to associate its presence with the clinical features. In addition, the levels of F. alocis as well as the total bacterial cells in the samples were also quantitated.
Design: One hundred teeth (50 PEI and 50 SEI) were included. Microbial samples were performed using sterile paper points and assessed by using nested PCR and quantitative Real Time PCR (qPCR). The prevalence of F.alocis in RCs from PEI and SEI were compared by chi-square analysis. Fisher´s exact test or Pearson Chi-square, when appropriate, was used to test associations between clinical and radiographic features and the presence of F. alocis. Significance level was set at 5%.
Results: F. alocis was detected in 23 and 28 (PEI) and 12 and 11 (SEI) RCs using Nested PCR and qPCR, respectively. Statistically significant associations were found between the presence of F. alocis and PEI, pain, wet canals, swelling, abscess and purulent exudate (P < 0.05). Total bacterial count was similar in both conditions (P > 0.05).
Conclusions: PEI harbour a significantly higher number of F. alocis than those with SEI. Filifactor alocis was significantly associated with clinical features in primary endodontic infections. Total bacterial count was similar in both clinical conditions.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.archoralbio.2020.104826 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Pathogenicity Requires TLR2 and the Oral Microbiome.
J Dent Res
May 2025
Department of Oral Immunology and Infectious Diseases, School of Dentistry, University of Louisville, Louisville, KY, USA.
Enrichment of the oral microorganism is strongly associated with the progression of periodontitis. However, is part of a complex dysbiotic microbial community, and the organism's direct pathogenic potential remains uncharacterized. Using the oral gavage model of experimental periodontitis, we revealed that promotes alveolar bone loss, as well as significant overexpression of proinflammatory markers associated with osteoclastogenesis and inflammation in oral tissues.
View Article and Find Full Text PDFSynergistic induction of PGE2 by oral pathogens and TNF promotes gingival fibroblast-driven stromal-immune cross-talk in periodontitis.
mBio
May 2025
Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland.
The interaction between pathogenic microorganisms and stromal cells, in particular fibroblasts, significantly contributes to the pathogenesis of many bacterially driven diseases. In periodontitis, oral pathogens penetrate the epithelial barrier and aggravate ongoing gingival inflammation by promoting the production of inflammatory mediators, such as prostaglandin E2 (PGE2). This study aimed to investigate the functional consequences of the interplay between oral pathogens and a pro-inflammatory environment in the activation of the PGE2 pathway in primary human gingival fibroblasts (GFs).
View Article and Find Full Text PDFIdentification of in Periodontal Biofilms using Polymerase Chain Reaction Technique: A Cross-Sectional Study.
J Pharm Bioallied Sci
December 2024
Department of Dental Surgery, Government Medical College, Omandurar Govt Estate, Chennai, Tamil Nadu, India.
Background And Aim: Contribution of organisms causing periodontal disease remains unclear. Strong association has emerged between the presence of gram-positive and periodontitis. Hence, we aimed to identify the presence of in periodontal biofilms through PCR technique in health and disease.
View Article and Find Full Text PDFPrevalence of the oral pathogen and its FtxA toxin related to clinical parameters and presence of .
Front Cell Infect Microbiol
May 2025
Department of Odontology, Umeå University, Umea, Sweden.
The Gram-positive organism is implicated in multiple oral diseases including periodontitis, and approximately 50% of known strains encode and produce a recently identified repeat-in-toxin (RTX) protein, FtxA, partly homologous to the leukotoxin. By assessing a longitudinal Ghanaian study population of adolescents, we recently identified a possible correlation between levels, gene carriage, and progression of clinical attachment loss (CAL). To extend knowledge on the possible significance of and its FtxA in periodontal disease, we have in the present work analyzed saliva samples in an independent cohort of periodontitis (n=156), collected at two private periodontal specialist practices in Perth, Western Australia.
View Article and Find Full Text PDFAn overview of the microbiota of the oral cavity of humans and non-human primates with periodontal disease: Current issues and perspectives.
Arch Oral Biol
April 2025
Pathology, Science in Microbiology, Oswaldo Cruz Institute, Brazil. Electronic address:
Objective: To provide a comprehensive summary of the available evidence on the oral microbiota of humans and non-human primates about the etiology of periodontal disease.
Design: An integrative literature review was conducted on 398 clinical and observational articles published between 2010 and 2024 using searches in the MEDLINE/PubMed, Virtual Health Library, and SciELO databases. After the screening, eligibility, data extraction, and methodological quality assessment, 21 studies were selected.