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Article Abstract
Two azobenzenesulfonamide molecules with thermally stable configurations resulting from fluorination of positions to the azo group are reported that can differentially regulate the activity of carbonic anhydrase in the and configurations. These fluorinated probes each use two distinct visible wavelengths (520 and 410 or 460 nm) for isomerization with high photoconversion efficiency. Correspondingly, the isomer of these systems is highly stable and persistent (as evidenced by structural studies in solid and solution state), permitting regulation of metalloenzyme activity without continuous irradiation. Herein, we use these probes to demonstrate the visible light mediated bidirectional control over the activity of zinc-dependent carbonic anhydrase in solution as an isolated protein, in intact live cells and in zebrafish during embryo development.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063266 | PMC |
| http://dx.doi.org/10.1021/jacs.0c05383 | DOI Listing |
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