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http://dx.doi.org/10.2340/00015555-3538 | DOI Listing |
Microbiol Spectr
September 2025
Institute of Respiratory Health, Frontiers Science Center for Disease-related Molecular Network, West China Hospital, Sichuan University, Chengdu, China.
Efficient DNA delivery is essential for genetic manipulation of mycobacteria and for dissecting their physiology, pathogenesis, and drug resistance. Although electroporation enables transformation efficiencies exceeding 10⁵ CFU per µg DNA in and , it remains highly inefficient in many nontuberculous mycobacteria (NTM), including . Here, we discovered that NTM such as exhibit exceptional tolerance to ultra-high electric field strengths and that hypertonic preconditioning partially protects cells from electroporation-induced damage.
View Article and Find Full Text PDFPhytochemistry
September 2025
Équipe "Chimie des substances naturelles" BioCIS, CNRS, Université Paris-Saclay, 17, avenue des Sciences, 91400, Orsay, France. Electronic address:
Throughout the past decades, annonaceous plants have been of particular interest to the natural product community because of their therapeutic value and their richness in isoquinoline alkaloids. Taking advantage from our laboratory historical collection of these compounds, a MS/MS database of 322 isoquinolines and other metabolites from Annonaceae was implemented and named IQAMDB . The present report describes the dereplication of known alkaloids from stem barks of Greenwayodendron suaveolens (Engl.
View Article and Find Full Text PDFPLoS Pathog
September 2025
Centre for Molecular Inflammation Research (CEMIR), Norwegian University of, Science and Technology (NTNU), Trondheim, Norway.
Drosophila melanogaster (Drosophila) is one of the most extensively studied animal models we have, with a broad, advanced, and organized research community. Yet, Drosophila has barely been exploited to understand the underlying mechanisms of mycobacterial infections, which cause some of the deadliest infectious diseases humans are currently battling. Here, we identified mycobacterial genes required for the pathogen's growth during Drosophila infection.
View Article and Find Full Text PDFmLife
August 2025
Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), Shanghai Frontiers Science Center of Pathogenic Microorganisms and Infection, Shanghai Institute of Infectious Disease and Biosecurity, School of Basic Medical Sciences, Shanghai Medical College Fudan University Shanghai China.
Extractable glycolipids of mycobacteria, such as lipooligosaccharides (LOSs), play crucial roles in responding to environmental stress and modulating the host immune response. Although the biosynthesis of LOS is likely regulated at multiple levels to ensure proper composition of the cell wall, the key regulators remain unknown. In this study, we investigated B11, a conserved mycobacterial small RNA (sRNA), and found that it post-transcriptionally regulates LOS synthesis in .
View Article and Find Full Text PDFJAMA Dermatol
August 2025
Department of Dermatology, Tianjin Academy of Traditional Chinese Medicine Affiliated Hospital, Tianjin, China.