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This study reports the effects of seasonal variation on the total polyphenol and flavonoid content and the in vitro antioxidant activity of Secondatia floribunda A. DC. The extracts were prepared from the inner bark and heartwood of samples harvested in the 2015 to 2016. The total phenolic and flavonoid content was determined by specific qualitative tests. The in vitro antioxidant capacity was analyzed using the following tests: 1-1-diphenyl-2-picrylhydrazyl radical (DPPH), 2,2'-azinobis-3-ethylbenzenothiazoline-6-sulfonic acid (ABTS), ferric reducing antioxidant power (FRAP) and iron (Fe) chelating activity. The total polyphenol and total flavonoid content varied over the harvest period. The DPPH and ABTS tests revealed that in the dry season had the most potent in vitro antioxidant activity, although the extracts obtained during the rainy season presented the higher Fe chelating and Ferric reducing activities. In conclusion, the phenolic content and in vitro antioxidant activity are correlated, and both are influenced by seasonality.
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http://dx.doi.org/10.1016/j.foodchem.2020.126277 | DOI Listing |
Chem Biodivers
September 2025
Research Management Unit, Centre for Research and Enterprise, University of Cyberjaya, Persiaran Bestari, Cyber 11, Cyberjaya, Selangor, Malaysia.
Seaweeds are marine macroalgae that are rich in various secondary metabolites known to exhibit different biological activities such as anti-diabetic, anti-inflammatory, antioxidant, etc. This study aimed to determine the bioactive metabolites, as well as the antioxidant and anti-inflammatory activities of two red algae (Ceramium virgatum and Gracilaria corticata) and two green algae (Enteromorpha flexuosa and Ulva fasciata), which are prevalent in the coastal region of the Bay of Bengal. The total phenolic and flavonoid contents were determined using the Folin-Ciocalteu and aluminium chloride methods.
View Article and Find Full Text PDFPLoS One
September 2025
Laboratory of Molecular and Cellular Immunology, Institute of Molecular Biology, National Academy of Sciences, Yerevan, Armenia.
The short lifespan of polymorphonuclear neutrophils (PMNs) in vitro poses challenges, as their limited viability restricts functional assays and experimental manipulations. The HL-60 cell line serves as a valuable model for neutrophil-like differentiation, yet the functional relevance of ATRA- and DMSO-induced differentiation remains incompletely understood. In the present study, we aimed to characterize the differentiation potential of all-trans retinoic acid (ATRA) and dimethyl sulfoxide (DMSO) on HL-60 cells and compare their functionality with primary PMNs.
View Article and Find Full Text PDFACS Appl Mater Interfaces
September 2025
MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, School of Optoelectronic Science and Engineering, South China Normal University, No.55 West Zhongshan Avenue, Tianhe District, Guangzhou 510631, Guangdong, China.
While reactive oxygen species (ROS)-dependent chemodynamic therapy (CDT) and photodynamic therapy (PDT) hold promise for cancer treatment, their efficacy remains constrained by tumor microenvironment (TME) barriers: glutathione (GSH) overexpression, insufficient HO supply, and hypoxia. To address these limitations, we engineered a Trojan horse-inspired MnO-shelled CaO nanoreactor (CaO/MnO-Ce6-PEG) by employing a sequential TME reprogramming strategy, triggering a cascading ROS storm for enhanced CDT and PDT. The outer MnO layer first depletes GSH through redox conversion, exposing the CaO core hydrolysis, and subsequently providing HO for CDT and O for ameliorating hypoxia to boost Ce6-mediated PDT.
View Article and Find Full Text PDFInt J Vitam Nutr Res
August 2025
Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Dhaka, 1000 Dhaka, Bangladesh.
Diabetes mellitus (DM) is a chronic metabolic disorder characterized by persistent hyperglycemia and associated with severe complications, including cardiovascular diseases, neuropathy, nephropathy, and retinopathy. Although synthetic antidiabetic drugs are available, the side effects and limited long-term effectiveness of these medications highlight the urgent need for safer, more potent alternative therapies. L.
View Article and Find Full Text PDFJ Biochem Mol Toxicol
September 2025
Department of Molecular Biology and Genetics, Faculty of Science, Bartin University, Bartin, Turkey.
Schiff bases containing sulfonyl units are important compounds because of their potential biological properties in the therapeutical field. In this study, three novel ligands (L1, L2, and L3) containing the sulfonyl groups, a derivative of Schiff base, were synthesized, and their molecular structures were characterized by FT-IR, H-NMR, C NMR, and elemental analysis results. The antiproliferative activities of these Schiff base ligands were evaluated against human colon cancer (HT-29 and Caco-2) and mouse fibroblast (L929) cells by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method.
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