98%
921
2 minutes
20
The aim of the present study was to investigate the effect of kisspeptin-10 (Kp10) injection on semen characteristics, testosterone (T) production and sperm rheotaxis using microfluidic devices in immature ram. Computer-assisted sperm analysis (CASA) with controlled flow velocity was used to explore the kinetic parameters of sperm and positive rheotaxis (PR %). PR % was defined as the number of PR sperms over the number of motile sperms. Healthy Ossimi rams were randomly divided into two groups; a saline-treated control group and Kp10-treated one (5 µg/kg body weight). Treatments were given by intramuscular injection once a week for 1 month. After 1 month, the semen was collected and evaluated weekly for 6 weeks, while the blood samples were collected every 2 weeks for the next 8 weeks. Semen properties were significantly affected by Kp10 injection (p < .01). The Kp10 increased the volume, sperm concentration and percentages of live sperm compared with those of control. Additionally, sperm trajectories and rheotaxis get improved by the injection of Kp10 with time. Furthermore, kisspeptin improved the secretion of testosterone levels throughout the period of study. In conclusion, injections of the Kp10 had a positive impact on semen characteristics as well as improved sperm rheotaxis of Ossimi rams in subtropics.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.1111/rda.13613 | DOI Listing |
Reprod Domest Anim
September 2025
Department of Teaching Veterinary Clinical Complex, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India.
The present study was undertaken to assess the effect of kisspeptin supplementation (0.0, 5.0, 10.
View Article and Find Full Text PDFFront Reprod Health
August 2025
Department of Medical Laboratory Science, Afe Babalola University, Ado-Ekiti, Nigeria.
Background: Vitamin D, sometimes referred to as the "sunshine vitamin", is well-known for its role in maintaining bone health processes that are highly dependent on calcium regulation. However, there is an emerging wealth of evidence that this fat-soluble vitamin has an important role in male sexual health regarding erectile function, production of testosterone, and overall fertility. Due to the increased vitamin D deficiency rates in most populations, the implications of its deficiency on male sexual functions have gained great interest.
View Article and Find Full Text PDFCryobiology
September 2025
Laboratory of Teaching and Research in Pathology of Reproduction, Center of Biotechnology in Animal Reproduction, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo (USP), Pirassununga, SP, Brazil. Electronic address:
Sperm capacitation is a critical process for successful fertilization, involving multiple regulated cellular changes. On the other hand, cryopreservation induces membrane changes that can mimic capacitation, potentially leading to misinterpretation of sperm function. Distinguishing true capacitation from cryoinjury remains challenging, as both share surface markers despite involving distinct mechanisms and impacts on fertilization.
View Article and Find Full Text PDFReprod Domest Anim
September 2025
Department of Biological Sciences, State University of Santa Cruz (UESC), Ilhéus, Bahia, Brazil.
Characterising body and reproductive morphometry and their association with epididymal sperm quality can contribute to the conservation of sambar deer (Rusa unicolor). Five adult males maintained in captivity at the Getúlio Vargas Zoobotanical Park (Salvador, BA, Brazil) were captured, anaesthetised, and subjected to bilateral orchiectomy as part of a population-control strategy. Body measurements included head circumference, thoracic diameter, total length, withers height, and body weight.
View Article and Find Full Text PDFReprod Domest Anim
September 2025
School of Agriculture, Tarbiat Modares University, Tehran, Iran.
This experiment evaluated the effects of increasing MitoQ concentrations in semen extender on post-thaw quality of Holstein bull sperm, including motility, membrane integrity, antioxidant status and viability. Semen samples were collected, pooled and diluted with extender containing 0 (control), 5, 50, 500 and 1000 nM of MitoQ and frozen through the standard procedure. An increase in MitoQ supplementation positively influenced total and progressive motility, as well as average path velocity; however, these effects were not statistically significant until the concentration reached 50 nM.
View Article and Find Full Text PDF