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When a novel fluorescent probe made of BINOL and coumarin is excited at λ = 365 nm in a neutral buffer solution, it shows fluorescence enhancement at 465 nm in the presence of an amino acid with a very small difference between the two enantiomers. When excited at λ = 467 nm, it shows highly enantioselective fluorescence enhancement at 534 nm. This allows the determination of both concentration and enantiomeric composition of amino acids.
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http://dx.doi.org/10.1021/acs.orglett.9b03437 | DOI Listing |
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September 2025
Institute of Nanotechnology (INT), Karlsruhe Institute of Technology (KIT), Kaiserstraße 12, 76131, Karlsruhe, Germany.
Recently, metal-organic frameworks (MOFs) have shown high potential in the field of sensing. However, fluorescent-based detection with MOFs in solution needs complex pre-treatments and has stability issues, complicating measurements and handling for sensing applications. Here, an easy-to-handle and low-cost strategy is introduced to convert MOF-based sensing from solution to surface using scanning probe lithography.
View Article and Find Full Text PDFNucleosides Nucleotides Nucleic Acids
September 2025
School of Basic Medical Sciences, Yan'an University, Yan'an, China.
Live-cell imaging of intracellular proteins enables real-time observation of protein dynamics under near-physiological conditions, providing pivotal insights for both fundamental life science research and medical applications. However, due to limitations such as poor probe permeability and cytotoxicity associated with conventional antibody-based or genetically encoded labeling techniques, live-cell imaging remains a significant challenging. To address these limitations, here in this study, we developed and rigorously validated a novel aptamer-based fluorescent probe for real-time imaging of NEK9 kinase in living cells.
View Article and Find Full Text PDFJ Biomed Opt
September 2025
Fraunhofer Institute for Microelectronic Circuits and Systems IMS, Duisburg, Germany.
Significance: The spatial and temporal distribution of fluorophore fractions in biological and environmental systems contains valuable information about the interactions and dynamics of these systems. To access this information, fluorophore fractions are commonly determined by means of their fluorescence emission spectrum (ES) or lifetime (LT). Combining both dimensions in temporal-spectral multiplexed data enables more accurate fraction determination while requiring advanced and fast analysis methods to handle the increased data complexity and size.
View Article and Find Full Text PDFRSC Chem Biol
September 2025
Science for Life Laboratory, Department of Women's and Children's Health, Karolinska Institutet 17165 Solna Sweden
Labeling the plasma membrane for advanced imaging remains a significant challenge. For time-lapse live cell imaging, probe internalization and photobleaching are major limitations affecting most membrane-specific dyes. In fixed or permeabilized cells, many membrane probes either lose signal after fixation or fail to remain localized to the plasma membrane.
View Article and Find Full Text PDFChem Sci
September 2025
Institute of Optical Materials and Chemical Biology, Guangxi Key Laboratory of Electrochemical Energy Materials, School of Chemistry and Chemical Engineering, Guangxi University Nanning Guangxi 530004 China
As a cutting-edge super-resolution imaging technique, structured illumination microscopy (SIM) has been widely used in cell biology research, especially in the analysis of subcellular organelles and monitoring of their dynamic processes. Through multiple illumination and reconstruction processes, SIM breaks through the resolution limitations of traditional microscopes and can observe the fine structures within cells in real time with nanoscale resolution. This provides strong technical support for in-depth analyses of molecular mechanisms, organelle functions, signaling networks, and metabolic regulatory pathways within cells.
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