98%
921
2 minutes
20
Imbalance in extracellular ATP levels in brain tissue has been suggested as a triggering factor for several neurological disorders. Here, we describe the most sensitive and reliable technique for monitoring the ATP levels in mice cerebrospinal samples collected by cisterna magna puncture technique and quantified using a microplate reader.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.1007/978-1-4939-9717-6_17 | DOI Listing |
Eur J Dent
September 2025
Doctoral Program, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.
Although platelet-rich plasma (PRP) has demonstrated considerable regenerative potential in regenerative endodontic treatment, its clinical efficacy may be limited by the rapid degradation of its bioactive components, leading to inconsistent outcomes. To overcome this challenge, the present study explores the use of nano-sized exosomes derived from PRP-a novel designated as PRP exosomes (PRP-Exo)-as a more stable and targeted biomolecular delivery system to promote odontogenic differentiation within the dentin-pulp complex. The primary objective is to investigate the expression of key odontogenic markers, transforming growth factor-β1 (TGF-β1) and Dentin Sialophosphoprotein (DSPP), in human dental pulp stem cells (hDPSCs) following PRP-Exo treatment.
View Article and Find Full Text PDFProc Natl Acad Sci U S A
September 2025
Department of Ecology and Evolutionary Biology, Yale University, New Haven, CT 06520.
A frequent goal of phage biology is to quantify how well a phage kills a population of host bacteria. Unfortunately, traditional methods to quantify phage success can be time-consuming, limiting the throughput of experiments. Here, we use theory to show how the effects of phages on their hosts can be quantified using bacterial population dynamics measured in a high-throughput microplate reader (automated spectrophotometer).
View Article and Find Full Text PDFhas long been used in folk medicine to treat a variety of ailments, including diabetes, skin disorders, dropsy, cuts, wounds, ulcers, fever, and blood disorders, etc., which are generally categorized under the complications of diabetes mellitus. Various species of this genus have also been verified to possess strong anti-diabetic activity.
View Article and Find Full Text PDFJ Inflamm Res
August 2025
Department of Human Anatomy, School of Basic Medicine, Guizhou Medical University, Gui'an New District, People's Republic of China.
Purpose: Investigate the effects of repetitive Trans-Spinal Magnetic Stimulation (rTSMS) on motor function recovery and the underlying mechanisms in mice after spinal cord injury.
Methods: rTSMS was applied both in vivo and in vitro, Motor function was evaluated by the Basso Mouse Scale (BMS), grid walking errors, and Motor Evoked Potentials (MEPs). Cell viability, oxidative stress markers, and key proteins including AQP4, Bax, Bcl-2, cleaved caspase-3, inflammatory cytokines, and NLRP3 inflammasome components were analyzed.
J Genet Eng Biotechnol
September 2025
Department of Therapeutic Chemistry, Institute of Pharmaceutical and Drug Industries Research, the National Research Centre, Dokki, Giza 12311, Egypt; Research Group Immune- and Biomarkers for Infection, the Centre of Excellence for Advanced Sciences, the National Research Centre, Giza 12311, Egypt;
Background: SARS-CoV-2 enters human cells via angiotensin-converting enzyme 2 (ACE2). In silico experiments also demonstrated possible SARS-CoV-2 binding to dipeptidyl peptidase 4 (DPP-4) during cell entry. Moreover, matrix metalloproteinase 2 (MMP-2) is involved in the SARS-CoV-2 cell fusion process.
View Article and Find Full Text PDF