Intramuscular Nerve Distribution of the Inferior Oblique Muscle.

: The intramuscular nerve distribution in the extraocular muscles is important for understanding their function. This study aimed to determine the intramuscular nerve distribution of the oculomotor nerve within the inferior oblique muscle (IO) using Sihler's staining.: Seventy-two IOs from 50 formalin-embalmed cadavers were investigated. The IO including its branch of the oculomotor nerve was finely dissected from its origin to its insertion point into the sclera. The total length of the muscle and its width were measured. The intramuscular nerve course was investigated after performing Sihler's staining, which is a whole-mount nerve-staining technique that stains the nerves while rendering other soft tissues either translucent or transparent.: The total length of the muscle and muscle width were 30.0 ± 2.8 mm (mean±standard deviation), 8.8 ± 1.2 mm, respectively. The oculomotor nerve enters the IO around the middle of the muscle and then divides into multiple smaller branches without distinct subdivisions. The intramuscular nerve distribution within the IO has a root-like arborization and supplies the entire width of the muscle. The Sihler's stained intramuscular nerve course (covering a length of 7.6 ± 1.2 mm) finishes around the distal one-third of the IO in gross observations.: Sihler's staining is a useful technique for visualizing the gross nerve distribution of the IO. This new information about the nerve distribution and morphological features will improve the understanding of the biomechanics of the IO.