Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Most human genes are associated with promoters embedded in non-methylated, G + C-rich CpG islands (CGIs). Not all CGIs are found at annotated promoters, however, raising the possibility that many serve as promoters for transcripts that do not code for proteins. To test this hypothesis, we searched for novel transcripts in embryonic stem cells (ESCs) that originate within orphan CGIs. Among several candidates, we detected a transcript that included three members of the micro-RNA family: and . Deletion of the CGI prevented expression of the precursor RNA and depleted the included miRNAs. Mice homozygous for this mutation were sub-viable and showed growth and other defects. The results suggest that despite the identity of their seed sequences, members of the miRNA family exert distinct functions that cannot be complemented by other members.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6660310 | PMC |
| http://dx.doi.org/10.3390/epigenomes3010007 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Multiomics analysis reveals the genetic and epigenetic features of high-risk NK cell-type chronic active EBV infection.
Blood
July 2025
Graduate School of Medicine, Kyoto University, Kyoto, Japan.
Chronic active Epstein-Barr virus (EBV) infection (CAEBV) is an orphan disease characterized by the proliferation and infiltration of EBV-infected T/natural killer (NK) cells into multiple organs. Although CAEBV is a heterogeneous disease with diverse clinical courses, its pathogenesis remains poorly understood. In this study, we explored the molecular mechanisms underlying CAEBV by performing a comprehensive multi-omics analysis, including genome, transcriptome, epigenome, and single-cell transcriptome and surface proteome analyses, of 65 CAEBV patients.
View Article and Find Full Text PDFUncovering a Novel Role of ROR1 in the Epigenetic Regulation of Tumor Suppressor Gene CREB3L1 in Triple-Negative Breast Cancer Cells.
Biomolecules
May 2025
Department of Biology, St. Joseph's University, Philadelphia, PA 19131, USA.
A characteristic of triple-negative breast cancer (TNBC) is the epigenetic regulation of tumor suppressor genes, leading to TNBC heterogeneity and treatment resistance in patients. TNBC exhibits high methylation rates, leading to the silencing of numerous tumor suppressor genes. DNA methyltransferase inhibitors (DNMTis) have shown limited clinical efficacy in TNBC treatment.
View Article and Find Full Text PDFSubtyping Burkitt Lymphoma by DNA Methylation.
Genes Chromosomes Cancer
April 2025
Institute of Human Genetics, Ulm University and Ulm University Medical Center, Ulm, Germany.
Burkitt lymphoma (BL) is an aggressive germinal center B-cell-derived malignancy. Historically, sporadic, endemic, and immunodeficiency-associated variants were distinguished, which differ in the frequency of Epstein-Barr virus (EBV) positivity. Aiming to identify subgroups based on DNA methylation patterns, we here profiled 96 BL cases, 17 BL cell lines, and six EBV-transformed lymphoblastoid cell lines using Illumina BeadChip arrays.
View Article and Find Full Text PDFMECP2 Variants in Males: More Common than Previously Appreciated.
Pediatr Neurol
December 2024
Heersink School of Medicine, University of Alabama at Birmingham, Birmingham, Alabama. Electronic address:
Article Synopsis
- The study aims to analyze the ages and genetic MECP2 variants of recently identified males, laying the groundwork for further investigation into their clinical characteristics.
- Genetic data were collected from a parent group, focusing on whether MECP2 variants were newly developed or inherited, as well as the prevalence of mosaicism among those meeting Rett syndrome criteria.
- Out of 59 males examined, the majority had de novo variants, and findings emphasize the necessity for improved diagnostic processes and equitable access to therapeutic options for those with MECP2 variants.
Integration of multi-omics data revealed the orphan CpG islands and enhancer-dominated -regulatory network in glioma.
iScience
October 2024
Department of Neurosurgery, The Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China.
Article Synopsis
- The study investigates the role of orphan CpG islands (oCGIs) in the transcriptional network related to glioma, a type of brain cancer with poor prognosis.
- By integrating multi-omics data, researchers found that oCGIs and enhancers play a crucial role in regulating target genes, particularly in a specific cell cluster (C2) that exhibits high proliferation.
- The upregulation of oCGIs and enhancer-related genes in this cluster contributes to glioma cells' resistance to treatments like radiotherapy and chemotherapy, offering new insights for potential therapeutic strategies.