Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
We developed a fully-automated dicentric chromosome assay (DCA) in multiwell plates. All operations, from sample loading to chromosome scoring, are performed, without human intervention, by the second-generation Rapid Automated Biodosimetry Tool II (RABiT-II) robotic system, a plate imager and custom software, FluorQuantDic. The system requires small volumes of blood (30 µl per individual) to determine radiation dose received as a result of a radiation accident or terrorist attack. To visualize dicentrics in multiwell plates, we implemented a non-classical protocol for centromere FISH staining at 37°C. The RABiT-II performs rapid analysis of chromosomes after extracting them from metaphase cells. With the use of multiwell plates, many samples can be screened at the same time. Thus, the RABiT-II DCA provides an advantage during triage when risk-based stratification and medical management are required for a large population exposed to unknown levels of ionizing radiation.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8567107 | PMC |
| http://dx.doi.org/10.1667/RR15266.1 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Quantification and Comparison of Different Biofilm Components from Methicillin-Susceptible Treated with Tranexamic Acid Using an In Vitro Model.
Microorganisms
August 2025
Department of Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Marañón, 28009 Madrid, Spain.
As we previously demonstrated that tranexamic acid (TXA), an antifibrinolytic, showed an antibacterial effect alone and in combination with vancomycin and gentamicin, we now wanted to analyze its own efficacy using new, different fluorescent staining reagents that target different components of the biofilm matrix and compare which one quantifies biofilm reduction better. A 10 cfu/mL suspension of the (ATCC29213) strain was placed into the wells of a 24-multiwell plate covered with glass slides coated with 10% poly-L-lysine under agitation for 24 h at 37 °C. After 3 washes with PBS, wells were treated with either TXA 10 mg/mL or sterile water and incubated for 24 h at 37 °C.
View Article and Find Full Text PDFProtocol for Quantifying γH2AX Foci in Irradiated Cells Using Immunofluorescence and Fiji Software.
Bio Protoc
August 2025
Department of Gynecologic Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Quantification of DNA double-strand breaks (DSBs) is critical for assessing genomic damage and cellular response to stress. γH2AX is a well-established marker for DNA double-strand breaks, but its quantification is often performed manually or semi-quantitatively, lacking standardization and reproducibility. Here, we present a standardized and automated workflow for γH2AX foci quantification in irradiated cells using immunofluorescence and a custom Fiji macro.
View Article and Find Full Text PDF[Evaluation of high-throughput detection technology for ubiquitination signals based on ThUBD].
Sheng Wu Gong Cheng Xue Bao
August 2025
Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, College of Chemistry and Materials Science, Hebei University, Baoding 071002, Hebei, China.
Ubiquitination is one of the most widely distributed, structurally diverse, and functionally important post-translational modifications for proteins in eukaryotic cells. At present, the methods for detecting ubiquitination signals mainly include immunological detection based on specific antibodies, mass spectrometry, and detection based on ubiquitin-binding domain (UBD), which together constitute a tool library for studying ubiquitination signals. Our team has previously developed a high-throughput detection technology based on an artificial tandem hybrid ubiquitin-binding domain (ThUBD), which achieves universal and highly sensitive detection of all polyubiquitin chain modification signals.
View Article and Find Full Text PDFAn Fluorescence Image Analysis Tool for Esterase Activity Quantification in : Using Calcein AM in Ecotoxicological Studies.
Environ Sci Technol
September 2025
Science of Life Laboratory, Department of Environmental Science, Stockholm University, 114 18 Stockholm, Sweden.
There is an increasing need for new approach methodologies (NAMs) to generate relevant ecotoxicological data. This study demonstrates the strengths of calcein AM, a highly sensitive fluorescent stain for esterase activity, in an automated image-based multiwell plate assay for detecting sublethal effects in . Sample processing and feeding conditions were optimized to ensure a uniform dye distribution.
View Article and Find Full Text PDFComparative analysis of 3D-culture techniques for multicellular colorectal tumour spheroids and development of a novel SW48 3D-model.
Sci Rep
July 2025
Cancer Genetics and Epigenetics group (CGE), Translational Program in Cancer Research (CARE), Germans Trias i Pujol Research Institute (IGTP), Badalona, Barcelona, Spain.
Colorectal cancer (CRC) is an important global health challenge, with nearly 2 million diagnosed cases and over 900,000 deaths annually despite therapeutic advancements. The high morbidity and mortality rates underscore the need for more efficient therapies. Three-dimensional (3D) cell culture models have emerged as more physiologically relevant alternatives to traditional two-dimensional (2D) models for drug screening and mechanistic studies.
View Article and Find Full Text PDF