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Article Abstract
Self-incompatibility (SI) is a widespread mechanism in angiosperms that prevents inbreeding by rejecting self-pollen. However, the regulation of the SI response in is not well understood. Here, we report that the M-locus protein kinase () , the functional homolog of in , controls SI in . We identified four paralogue genes in , including , , , and . Two transcripts of , and , were generated by alternative splicing. Tissue expression pattern analysis demonstrated that , especially , is highly expressed in reproductive organs, particularly in stigmas. We subsequently created RNA-silencing lines and CRISPR/Cas9-induced quadruple mutants of in SI line S-70. Phenotypic analysis revealed that SI response is partially suppressed in RNA-silencing lines and is completely blocked in quadruple mutants. These results indicate the importance of in regulating the SI response of . We found that the expression of SI positive regulators S-locus receptor kinase () and Arm-Repeat Containing 1 () are suppressed in mutant, whereas the self-compatibility (SC) element Glyoxalase I () maintained a high expression level. Overall, our findings reveal a new regulatory mechanism of in the SI of
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6651594 | PMC |
| http://dx.doi.org/10.3390/ijms20133303 | DOI Listing |
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