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Article Abstract
Rapid detection of low number of pathogenic bacteria in food is difficult. This study tested the filter-based loop-mediated isothermal amplification-lateral flow immunoassay (LAMP-LFA) method for rapid detection of pathogens in real food. O157:H7 was inoculated on 25 g of beef and the homogenized sample was filtered with 0.45 μm cellulose nitrate filter, and concentrated was recovered and DNA was extracted and analyzed by LAMP. LFA reaction was performed by hybridization of digoxygenin-labeled LAMP amplicon and biotinylated probe. The sensitivity of the filtered sample was 100 times more sensitive than that of the unfiltered sample. The total reaction time used for detection from sample preparation to confirmation of was within 3 h. These results suggest that the LAMP-LFA method can be used in real food systems as point-of-care testing for O157:H7 in beef.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6525692 | PMC |
| http://dx.doi.org/10.1007/s13197-019-03740-7 | DOI Listing |
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