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Aim: The aim of this study was to compare the effect of diode laser, endoActivator, and passive ultrasonics for smear layer removal at the apical third from root canals with 0.2% chitosan.
Materials And Methods: A total of 40 mandibular premolars were decoronated to establish a working length of 12 mm and shaped with ProTaper rotary files up to size F3. In Group A, canals were irrigated with 1 ml of 0.2% chitosan. In Group B, canals were initially irrigated with 0.8 ml of 0.2% chitosan and the remaining 0.2 ml was activated with diode laser. In Group C, canals were irrigated with 1 ml of 0.2% chitosan which was activated with endoActivator. In Group D, canals were irrigated with 0.2% chitosan and activated with passive ultrasonics. All samples were finally flushed with 3 ml of distilled water. The percentage of smear layer removal was analyzed with a scanning electron microscope examination at ×1000 and ×3000. Data were analyzed using Statistical Package for the Social Sciences version 16.0 (SPSS Inc., Chicago, IL, USA) at a significance level of < 0.05.
Results: The mean value for Group B when compared to Group C for the removal of smear layer was higher, but there was no statistically significant difference between the two groups ( < 0.068 and < 0.295). Both Group B and Group C showed a statistically significant difference ( < 0.001) when compared to Group A and Group D for the removal of smear layer.
Conclusion: Diode laser and endoActivator with 0.2% chitosan proved better in the removal of the smear layer when compared to passive ultrasonic irrigation.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6519194 | PMC |
http://dx.doi.org/10.4103/JCD.JCD_337_18 | DOI Listing |
J Dent
September 2025
Department of Restorative Dentistry, Faculty of Dentistry, Malaya University, Kuala Lumpur, Malaysia. Electronic address:
Objectives: to evaluate the effect of smear-layer deproteinization using papain gel and SPRG-adhesive on marginal-gap, anti-demineralization of enamel and dentin after chemical pH cycling and assess acid-base resistance zone (ABRZ) characteristics.
Methods: Cylindrical cavities were prepared cervically in thirty-two extracted premolars. Teeth were divided into two pretreatment groups (n=16); deproteinization with papain enzyme gel (Papacarie Due, Brazil) for 60 second, and no-deproteinization.
J Endod
August 2025
Department of Bioscience Research, College of Dentistry, University of Tennessee Health Science Center, Memphis, TN, USA; Department of Endodontics(,) College of Dentistry, The University of Tennessee Health Science Center, Memphis, Tennessee; Department of Physiology, College of Medicine, Universit
Introduction: To investigate the combined use of laser, nanobubble water (NB-HO), and calcium hydroxide (Ca(OH)) for endodontic disinfection, particularly in regenerative endodontic cases where maximum effectiveness is needed.
Methods: Canal of human teeth were prepared, sterilized and inoculated with Enterococcus faecalis (E. faecalis).
BMC Oral Health
August 2025
Department of Periodontology, Faculty of Dentistry, Gazi University, Ankara, Türkiye.
Background: Root surface biomodification (RSB) enhances tissue attachment by removing the smear layer, facilitating collagen fibril formation, and promoting clot formation and stabilization. This study aimed to evaluate the efficacy of injectable platelet-rich fibrin (i-PRF), an autologous blood product, as a potential adjunct to ethylenediaminetetraacetic acid (EDTA) for RSB in gingival fibroblast attachment and proliferation in vitro.
Methods: Dentin discs (4 mm in diameter) underwent root surface debridement to remove damaged cementum.
Shanghai Kou Qiang Yi Xue
June 2025
Department of Stomatology, Chifeng University Affiliated Hospital. Chifeng 024000, China. E-mail:
Purpose: To evaluate the effects of Er:YAG laser combined with NaClO on the removal of root canal taint layer, calcium hydroxide clearance and dentin microhardness.
Methods: A total of 318 single-rooted extracted teeth collected from March 2021 to January 2024 were selected, the 13 mm root was taken and mechanically prepared and randomly divided into three groups, with 106 teeth in each group. The control group was rinsed with NaClO, the experimental group 1 was rinsed with NaClO combined with ultrasonic washing, and the experimental group 2 was treated with Er:YAG laser on the basis of NaClO.
J Conserv Dent Endod
August 2025
Department of Orthodontics and Dentofacial Orthopedics, Bharati Vidyapeeth (Deemed to be University) Dental College and Hospital, Pune, India.
Aim: The aim of the study was to evaluate the antimicrobial properties of calcium hydroxide (CH)-loaded polylactic-co-glycolic acid (PLGA) nanoparticles (NPs) and CH when used as an intracanal medicament (ICM).
Methods: Adhering guidelines of the Preferred Reporting Items for Systematic Reviews and Meta-analysis, the review was registered in the Prospective Registration of Systematic Review-CRD42023454265. Databases from January 2000 to December 2023 were examined for studies evaluating the various properties of CH-loaded PLGA NPs compared to conventional ICM-CH.