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Article Abstract
mediated method was used to transform Indian elite wheat genotype HD2894 with herbicide-tolerant - (5-enolpyruvylshikimate-3-phosphate synthase) gene. The apical meristems of germinated seeds were targeted for introgression of transgene. The obtained T plants were screened by spraying 1% glyphosate and only positive transformants survived. The presence of transgene was also confirmed by PCR and Southern hybridization. Using this method, 3.07% transformation rate was observed. To identify transgenic lines carrying stably integrated - gene, the transgenic populations were screened in T generation using 1% glyphosate and lines with 100% survival were considered as homozygous. No significant morpho-physiological variations were observed within the transgenic lines as compared to non-transgenic plants. The present study resulted in herbicide-tolerant transgenic wheat and provides a valuable tool for development of wheat genetic transformation.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6470228 | PMC |
| http://dx.doi.org/10.1007/s13205-019-1708-6 | DOI Listing |
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