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strain MN05-02 is a UV-resistant bacterium isolated from a manganese deposit in the Sonoran Desert, Arizona, USA. The LD of this strain is 123 Jm, which is twice that of , and therefore can be a useful resource for comparative study of UV resistance and the role of manganese on this phenotype. Its complete genome is comprised of a chromosome of 3,488,433 bp and a plasmid of 154,991 bp. The chromosome contains 3,430 putative genes, including 3,366 protein coding genes, 52 tRNA and 12 rRNA genes. Carotenoid biosynthesis operon structure coded within the genome mirrors the characteristic orange-red pigment this bacterium produces, which presumably partly contribute to its UV resistance.
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http://dx.doi.org/10.7150/jgen.32194 | DOI Listing |
J Bacteriol
September 2025
Wadsworth Center, New York State Department of Health, Albany, New York, USA.
Prokaryotic genomes are gene-dense, so genes in the same orientation are often separated by short intergenic sequences or even overlap. Many mechanisms of regulation depend on open reading frames (ORFs) being spatially close to one another. Here, we describe one such mechanism, translational coupling, where translation of one gene promotes translation of a co-oriented neighboring gene.
View Article and Find Full Text PDFAppl Environ Microbiol
September 2025
College of Life Sciences, Northwest Normal University, Lanzhou, China.
Nitrogen leaching is a major pathway of nitrogen fertilizer loss. Although arbuscular mycorrhizal (AM) fungi are known to reduce nitrogen leaching by improving plant nitrogen uptake, the soil-based mechanisms remain unclear. A pot experiment was conducted using a randomized complete block design, with four nitrogen levels (0, 3.
View Article and Find Full Text PDFJ Microbiol Biol Educ
September 2025
University of California Riverside, Riverside, California, USA.
DNA literacy is becoming increasingly essential for navigating healthcare, understanding pandemics, and engaging with biotechnology-yet genomics education remains limited at the secondary level of education. We present a modular, hands-on curriculum designed for high school and early undergraduate students (ages 14-21) that introduces key genomics concepts through an experiment on fermentation, a process that is key to food preservation and medicine. Students follow a complete scientific process: exploring what DNA is and how microbial succession works, analyzing real DNA sequencing data, and writing a formal scientific report.
View Article and Find Full Text PDFJ Virol
September 2025
Laboratory of Virology, Wageningen University & Research, Wageningen, the Netherlands.
Vertebrate animals and many small DNA and single-stranded RNA viruses that infect vertebrates have evolved to suppress genomic CpG dinucleotides. All organisms and most viruses additionally suppress UpA dinucleotides in protein-coding RNA. Synonymously recoding viral genomes to introduce CpG or UpA dinucleotides has emerged as an approach for viral attenuation and vaccine development.
View Article and Find Full Text PDFJ Genet
September 2025
College of Life Sciences, Nanjing Forestry University, Nanjing 210037, People's Republic of China.
The family Syngnathidae includes seahorses, sea dragons, and pipefishes. We sequenced the complete mitochondrial DNA (mtDNA) genome of the belly pipefish, Bleeker, 1849. The genome is 16,646-bp long, and includes the standard complement for bony fishes of 13 protein-coding genes, 22 tRNA genes, two rRNA genes, and a control region, in the same order and strand distribution as other syngnathids.
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