Diversity among Pomegranate Varieties in Chilling Tolerance and Transcriptome Responses to Cold Storage.

J Agric Food Chem

Department of Fruit Tree Sciences , Agricultural Research Organization (ARO), Newe Ya'ar Research Center, Post Office Box 1021, Ramat Yishay 30095 , Israel.

Published: January 2019


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Article Abstract

We found great variability in chilling tolerance among 84 pomegranate varieties from the Newe Ya'ar collection; among them, 'Ganesh' was chilling-sensitive, whereas 'Wonderful' was relatively chilling-tolerant. To evaluate the different molecular responses of these varieties to cold storage, we analyzed the transcriptomic changes in the inner membrane tissues of 'Ganesh' and 'Wonderful' fruit after 2 weeks of cold storage at 1 °C. By functional categorization of the differentially expressed transcripts using MapMan, we found that many transcripts related to various pathways, such as jasmonic acid biosynthesis and signaling, galactinol, raffinose, phenol, and phenylpropanoid biosynthesis, calcium and mitogen-activated protein kinase signaling, lipid metabolism, and various transcription factors and heat-shock proteins, have been massively upregulated in 'Wonderful' but not in 'Ganesh' fruit. Thus, it is suggested that these pathways most likely participate in imparting chilling tolerance in pomegranate fruit.

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http://dx.doi.org/10.1021/acs.jafc.8b06321DOI Listing

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