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Article Abstract
N -Methyladenosine, an abundant chemical modification in mRNA, plays crucial roles in regulating gene expression and biological processes. Research on mA and its functions has progressed rapidly in the past few years, aided substantially by advances in high-throughput sequencing-based methods to profile mA along the transcriptome. We present here a protocol for mA crosslinking immunoprecipitation sequencing (mA-CLIP-seq), which profiles mA on mRNA at high resolution from as little as 1 μg of poly(A)-selected mRNA.
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| http://dx.doi.org/10.1007/978-1-4939-8808-2_5 | DOI Listing |
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