Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
In this study, an immuno-magnetic bead (IMB)-based assay was developed to simultaneously detect , and spp. and was tested in four animal-derived foods: beef, ham, egg, and ricotta cheese. The IMB-based assay exhibited good specificity by binding to five serotypes [capture efficiency (CE) average (avg.) 90.4%], five strains (CE avg. 91.4%), and five serotypes (CE avg. 95.4%) but not binding to non-target bacteria (CE<10%). Furthermore, the assay detected all three pathogens with a detection limit of 10 CFU/g without the need for enrichment or additional platforms. Since the results demonstrated that the IMB-based assay can effectively separate and enrich target bacteria from a variety of animal-derived food matrixes, the assay exhibits good pecificity for potential use in providing rapid, immunological, presumptive identification of pathogenic bacteria.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6131368 | PMC |
| http://dx.doi.org/10.5851/kosfa.2018.e11 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Asymmetric volume-mediated buffer control overcomes sensitivity limits in one-pot RAA-CRISPR/Cas12a visual detection.
Anal Bioanal Chem
September 2025
Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Military Medical Sciences Academy, Tianjin, 300050, China.
Rapid, low-cost, and visual nucleic acid detection methods are highly attractive for curbing colistin resistance spread through the food chain. CRISPR/Cas12a combined with recombinase-aided amplification (RAA) offers a one-pot, aerosol-free approach for visual detection. However, traditional one-pot systems often run Cas12a trans-cleavage in a buffer suitable for RAA, thus limiting Cas12a cleavage efficiency.
View Article and Find Full Text PDFCharacterization and Antimicrobial Efficacy of a Bacteriophage Targeting Multidrug-Resistant .
ACS Infect Dis
September 2025
Animal-Derived Food Safety Innovation Team, College of Veterinary Medicine, Anhui Agricultural University, Hefei 230036, China.
The emergence of multidrug-resistant (MDR) poses a significant threat to global public health, necessitating alternative therapeutic strategies. In this study, we isolated and characterized a novel lytic bacteriophage (phage), vB_EcoM_51, from poultry farm sewage and evaluated its potential against MDR . Transmission electron microscopy revealed that the phage exhibits morphological features typical of the family, including a polyhedral head (∼66.
View Article and Find Full Text PDF7-ketocholesterol as a critical oxysterol: Impact on human health and safety in food systems.
J Steroid Biochem Mol Biol
September 2025
Biochemistry and Phytochemistry Research Division, Jubilee Centre for Medical Research, Thrissur, Kerala 680005, India. Electronic address:
7-Ketocholesterol (7-KC) is a biologically active oxysterol formed through the oxidation of cholesterol, predominantly under conditions of oxidative stress. It is generated both enzymatically in specific tissues such as the brain and liver, and non-enzymatically via reactive oxygen species (ROS), especially in aging tissues and heat-processed animal-derived foods. 7-KC exerts multifaceted effects on human health, extending beyond lipid metabolism to disrupt glucose and amino acid utilization, impair mitochondrial function, and provoke endoplasmic reticulum (ER) stress.
View Article and Find Full Text PDFIdentification, and characterization of Escherichia coli O157:H7 from animal-derived foods in Hawassa and Yirgalem, Sidama Region, Ethiopia.
J Food Prot
September 2025
Faculty of Veterinary Medicine, Hawassa University, Hawassa Ethiopia, P.O.Box. 05.
Escherichia coli O157:H7 is a significant foodborne pathogen with global public health implications. This study, conducted from December 2022 to July 2023 in Hawassa and Yirgalem, Sidama Region, Ethiopia, assessed the prevalence, molecular identification, and antimicrobial resistance of E. coli O157:H7 in animal-derived foods.
View Article and Find Full Text PDFAdvances in methods for rapid detection of Staphylococcus aureus and methicillin-resistant S. aureus.
Anal Sci
September 2025
School of Animal Husbandry and Veterinary Medicine, Jiangsu Vocational College of Agriculture and Forestry, Jurong, 212400, People's Republic of China.
Staphylococcus aureus (S. aureus) and methicillin-resistant S. aureus (MRSA) are important pathogens that are closely associated with hospital-acquired and community-acquired infections.
View Article and Find Full Text PDF