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The phaeophyte macroalgae is under investigation as a cultivation crop within its native range in SE Asia, alongside other members of the genus. During the critical hatchery phase, germlings are grown to ≥ several millimeters ready for outplanting. By optimising the growth medium and twine substrate used for the germling attachment, hatcheries can become more efficient and cost-effective. An 8-week replicated laboratory experiment investigated these factors. It found that adding 0.125 mL L of saturated germanium dioxide during the first week increased mean germling size by 23% ( < 0.005), whereas additional nutrients in the form of F/2 medium made no difference ( > 0.05). Six twine substrates were also tested: jute, cotton, polyamide/cotton, polyester, polyvinyl alcohol and polypropylene. grew similarly well on all, although attachment success during the first week was highest on the rougher natural fibres, particularly jute. A negative density-dependent effect of germling density on growth was seen across all materials, with the highest growth seen on the materials with the lowest germling density. Jute is recommended as a highly suitable substrate for hatchery cultivation in this species, although the initial density should be carefully controlled to prevent intraspecific competition.
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http://dx.doi.org/10.1007/s10811-018-1459-5 | DOI Listing |
Front Microbiol
November 2020
Department of Microbiology, University of Innsbruck, Innsbruck, Austria.
is a mycoparasitic fungus used as biological control agent to protect plants against fungal pathogens. Successful biocontrol is based on the perception of signals derived from both the plant symbiont and the fungal prey. Here, we applied three different chemotropic assays to study the chemosensing capacity of toward compounds known or suspected to play a role in the mycoparasite/plant or host/prey fungal interactions and to cover the complete spectrum of developmental stages.
View Article and Find Full Text PDFBMC Biol
August 2020
Plant Pathology Group, Institute of Integrative Biology, ETH Zürich, 8092, Zürich, Switzerland.
Background: The ability of fungal cells to undergo cell-to-cell communication and anastomosis, the process of vegetative hyphal fusion, allows them to maximize their overall fitness. Previous studies in a number of fungal species have identified the requirement of several signaling pathways for anastomosis, including the so far best characterized soft (So) gene, and the MAPK pathway components MAK-1 and MAK-2 of Neurospora crassa. Despite the observations of hyphal fusions' involvement in pathogenicity and host adhesion, the connection between cell fusion and fungal lifestyles is still unclear.
View Article and Find Full Text PDFMethods Mol Biol
March 2021
Department of Agriculture. Food and Environment, University of Pisa, Pisa, Italy.
An in vivo whole-plant bi-dimensional experimental system has been devised and tested with different host plants, in order to obtain extraradical mycelium (ERM) produced by different arbuscular mycorrhizal fungi (AMF). In this system, a host plant germling is inoculated with AMF to establish mycorrhizal symbiosis, and, after colonization, newly formed extraradical hyphae and spores are removed. Then the mycorrhizal root system is wrapped in a nylon net and placed between membranes in a Petri dish, allowing ERM to grow on the membrane surface.
View Article and Find Full Text PDFPeerJ
July 2019
Department of Life Sciences, University of Trieste, Trieste, Italy.
Front Microbiol
March 2019
Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley CA, United States.
Cell-to-cell communication and cell fusion are fundamental biological processes across the tree of life. Survival is often dependent upon being able to identify nearby individuals and respond appropriately. Communication between genetically different individuals allows for the identification of potential mating partners, symbionts, prey, or predators.
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