Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Recently, next generation sequencers(NGS)became prevalent and enable us possible to comprehensively analyze the entire human microbiome community structures including difficult-to-culture microbes. In this review, we introduce the NGS-based analytical methods of human microbiome, called "meta-16S analysis" and "metagenomic analysis", then show several fundamental data basing these analyses. Furthermore, we also introduce the importance of database improvement used for analysis and the DNA preparation method from human samples.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Multi-Omics Analysis Revealed Characterization of Gastric Microbiome and Metabolome in Helicobacter pylori-Induced Progression of MASLD.
Helicobacter
September 2025
Department of Gastroenterology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Background: Several clinical studies have demonstrated that Helicobacter pylori (Hp) infection may exacerbate the progression of Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD); however, the underlying mechanisms remain unclear. This study aims to investigate the characterization of the gastric microbiome and metabolome in relation to the progression of MASLD induced by Hp infection.
Methods: We established a high-fat diet (HFD) obese mouse model, both with and without Hp infection, to compare alterations in serum and liver metabolic phenotypes.
Diet-microbiome synergy: unraveling the combined impact on frailty through interactions and mediation.
Nutr J
September 2025
Department of Geriatric, The First Hospital of China Medical University, No. 155 Nanjing North Street, Heping Ward, Shenyang, 110001, China.
Objective: This study analyzed data from the US population to examine how oral microbiome diversity and diet quality individually and synergistically affect frailty.
Methods: This study included 6,283 participants aged 20 years or older from the 2009-2010 and 2011-2012 NHANES cycles. A frailty index (FI) consisting of 36 items was developed, with items related to nutritional status excluded.
Gut Microbes and Inflammation: Their Role in Spinal Cord Injury Progression and Secondary Damage.
Curr Microbiol
September 2025
Department of Spine Surgery, Honghui Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi, China.
This review article describes recent research advances in the relationship between spinal cord injury (SCI) and the gut microbiota and each other's inflammatory response. SCI is a serious neurological disease that directly damages physiological function. Recent studies have shown that SCI significantly affected the composition and function of the gut microbiota, and even caused intestinal inflammation.
View Article and Find Full Text PDFThe Interplay Between Lifestyle and Oral/Faecal Microbial Profiles Among Periodontal Disease Patients: A Cross-Sectional Study.
J Clin Periodontol
September 2025
Department of Oral and Maxillofacial Surgery and Periodontology, Ribeirao Preto School of Dentistry, University of Sao Paulo (USP), Ribeirao Preto, Brazil.
Aim: To characterise periodontal and faecal microbiomes of individuals with periodontal health (PH) and diseases, and evaluate associations with periodontal, sociodemographic, anthropometric, nutritional and lifestyle factors.
Materials And Methods: Dental biofilm and faecal samples from individuals (n = 24/group) with PH, gingivitis (GG) and periodontitis (PE) were sequenced (16S rRNA). Anthropometric data and questionnaires on demographics, lifestyle, diet and intestinal habits were collected.
Multidimensional Research on Hair Loss in Young Chinese Females With Oily Scalps.
J Cosmet Dermatol
September 2025
School of Light Industry Science and Engineering, Beijing Technology and Business University, Beijing, People's Republic of China.
Background: In recent years, the problem of female alopecia has been increasing and has shown a trend toward youthfulness. However, there are fewer studies on young female alopecia in the existing literature.
Aim: We aimed to study the possible causes of hair loss in young Chinese females aged 18-35 with oily scalps.