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The NanoAptamer assay is a bisphenol A (BPA) quantification method that uses magnetic beads, quantum dot nanoparticles, and a BPA-specific aptamer. In this study, screening of various consumer and household products for BPA was demonstrated utilizing the NanoAptamer assay. First, the experimental conditions suitable for BPA detection using the NanoAptamer assay were examined in terms of incubation time, temperature, and buffer composition. The range of BPA quantification via the NanoAptamer assay was determined to be 0.005-1000 ng/mL of BPA. The selectivity was confirmed by detecting BPA in an analog mixture containing bisphenol S and bisphenol F. Finally, a leaching experiment using 20 consumer and household products classified into 4 categories was performed to demonstrate the capability of the NanoAptamer assay for BPA detection. The experiment was validated by high-performance liquid chromatography analysis (correlation coefficient, r = 0.99).
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http://dx.doi.org/10.1016/j.chemosphere.2018.07.125 | DOI Listing |
J Photochem Photobiol B
March 2022
Nanotechnology Laboratory, Nano Smart Science Institute, Tehran, Iran.
Ag doped SnO Nanostructure and immobilized on hyperbranched polypyrrole is investigated in this project. The product was synthesized by the hydrothermal synthesis method. The surface and structural characteristics of the product was studied by different instrumental analysis.
View Article and Find Full Text PDFInt J Nanomedicine
March 2020
Shaanxi Institute of Pediatric Diseases, Xi'an Children's Hospital, Xi'an, Shaanxi 86-710003, People's Republic of China.
Background: The restoration and repair method in the clinic of delayed fracture healing and non-union after comminuted fractures are urgently needed to improve the prognosis of patients. The recruitment of endogenous stem cells has been considered a promising approach in bone defect repair.
Propose: The aim of this study was to generate a de novel MSCs aptamer and developed the first, feasible, economical, bio-compatible, and functional MSCs aptamer-directed nanoparticles without complex manufacture to recruit mesenchymal stem cells (MSCs) for bone defect regeneration.
Analyst
September 2018
Department of Chemistry, Carleton University, 1125 Colonel By Drive, Ottawa, ON K1S 5B6, Canada.
Nano-aptamer probes were prepared and used in lateral flow colorimetric assays for the detection of Ochratoxin A (OTA). In this study, two approaches were examined using 5'-biotin-modified OTA aptamers and silver or gold nanoparticles (AgNP or AuNP). The first method used an "adsorption-desorption" approach wherein aptamers were adsorbed onto the metal nanoparticle surface.
View Article and Find Full Text PDFChemosphere
November 2018
Department of Environmental Science and Engineering, Ewha Womans University, Seoul 03760, Republic of Korea. Electronic address:
The NanoAptamer assay is a bisphenol A (BPA) quantification method that uses magnetic beads, quantum dot nanoparticles, and a BPA-specific aptamer. In this study, screening of various consumer and household products for BPA was demonstrated utilizing the NanoAptamer assay. First, the experimental conditions suitable for BPA detection using the NanoAptamer assay were examined in terms of incubation time, temperature, and buffer composition.
View Article and Find Full Text PDFACS Appl Mater Interfaces
May 2017
Department of Environmental Science and Engineering, Ewha Womans University, Seoul 03760, Republic of Korea.
For the sensitive quantification of bisphenol A (BPA), we have developed NanoAptamer assay, which employs aptamer and complementary signaling DNA, a set of quantum dots (QD), and magnetic beads (MBs). Signaling DNA-QD was tethered to MB-QD via the aptamer. The affinity of the aptamer to BPA resulted in the release of the signaling DNA-QD from the complex and hence the corresponding decrease in the QD fluorescence measurement signal.
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