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Article Abstract
is a close relative of that can cause systemic tuberculosis-like infections in ectotherms and skin infections in humans. Sliding motility correlates with biofilm formation and virulence in most bacteria. In this study, we used a sliding motility assay to screen 2,304 transposon mutants of NTUH-M6885 and identified five transposon mutants with decreased sliding motility. Transposons that interrupted the type VII secretion system (T7SS) ESX-1-related genes, (), (), and (), were present in 3 mutants. We performed reverse-transcription polymerase chain reaction to verify genes from to , which were found to belong to a single transcriptional unit. Deletion mutants of , , (), and () displayed significant attenuation regarding sliding motility and biofilm formation. NTUH-M6885 possesses a functional ESX-1 secretion system. However, deletion of or resulted in slightly decreased secretion of EsxB (which is also known as CFP-10). Thus, the ESX-1 secretion system mediates sliding motility and is crucial for biofilm formation. These data provide new insight into biofilm formation.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5988883 | PMC |
| http://dx.doi.org/10.3389/fmicb.2018.01160 | DOI Listing |
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