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In the preceding article "Top Down Tandem Mass Spectrometric Analysis of a Chemically Modified Rough-Type Lipopolysaccharide Vaccine Candidate" by Oyler et al., an error in the J5 E. coli LPS chemical structure (Figs. 2 and 4) was introduced and propagated into the final revision.
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http://dx.doi.org/10.1007/s13361-018-1960-8 | DOI Listing |
Biomed Chromatogr
October 2025
Department of Pharmaceutical Analysis, Pharmacy School, Shenyang Pharmaceutical University, Shenyang, China.
A rapid and specific liquid chromatography-tandem mass spectrometry method with a wide linear range was developed and validated for the simultaneous quantification of Vitamin K1 (VK1) trans- and cis- isomers in human plasma. Bovine serum albumin solution (15%) served as a surrogate matrix for preparing the calibrators to establish the quantitative curves. After liquid-liquid extraction, VK1 trans- and cis- isomers in plasma samples were separated on a ChromCore C30 column (15 cm × 4.
View Article and Find Full Text PDFBiomed Chromatogr
October 2025
College of Medicine, Lishui University, Lishui, China.
Saikosaponin A (SSa) is an oleanane type triterpenoid saponin isolated from Radix Bupleuri (Bupleurum chinense DC). While SSa has demonstrated significant pharmacological activities including anti-inflammatory, antioxidant, and antidepressant effects, its pharmacokinetic profile remains poorly characterized. This study developed and validated a sensitive LC-MS/MS method for quantifying SSa in rat plasma.
View Article and Find Full Text PDFJ Ethnopharmacol
September 2025
The First Hospital of Hunan University of Chinese Medicine, Changsha 410007, China; Key Laboratory of Research and Transformation on Prevention and Treatment of Major Diseases in Internal Medicine of Traditional Chinese Medicine, Ministry of Education, Changsha 410007, China. Electronic address: mic
Ethnopharmacological Relevance: Primary dysmenorrhea (PD) is common and has a major impact on women's daily activities and quality of life. Wenjing Decoction (WD), a classic Chinese medicine formula, has been widely used for thousands of years in China to treat PD. However, the key pharmacodynamic substances in WD responsible for its anti-dysmenorrhea efficacy are still unclear.
View Article and Find Full Text PDFFish Shellfish Immunol
September 2025
College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungnam National University, Yuseong-gu, Daejeon 34134, Republic of Korea. Electronic address:
Extracellular vesicles (EVs) or their sub types, such as exosomes are valuable nano-biomolecules for immunotherapeutic, drug delivery, and diagnostic purposes. Freshwater and marine fish, including olive flounder (Paralichthys olivaceus), are highly susceptible to the contagious Viral hemorrhagic septicemia virus (VHSV). In this study, we aimed to determine how infection alters the biological responses by analyzing the proteomic profiles of plasma-derived exosomes from phosphate buffered saline (PBS) injected (PBS-Exo) and VHSV challenged (VHSV-Exo) olive flounders at the initial stages infection.
View Article and Find Full Text PDFLeg Med (Tokyo)
August 2025
Department of Legal Medicine, Hamamatsu University School of Medicine, 1-20-1 Handayama, Hamamatsu 431-3192, Japan.
Reports on the quantification of fluvoxamine (FLV) in human tissues have been quite limited, although FLV has been used as an antidepressant since 1986. Fluvoxamine acid (FLA) was shown to be the major metabolite of FLV in human urine in 1983, but its quantification is also limited to only three works using human plasma. The existence of desmethyl fluvoxamine (FLD) in human specimens was recently reported in 2025; therefore, its quantification has not yet been performed.
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