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In this Letter, we show how to obtain high-contrast wide-field evanescent wave illuminated subdiffraction imaging through controlling nanoscale light-matter interaction. The light coupling, propagation, and far-field imaging processes show strong polarization selectivity and film quality dependence, which is used to improve the image-contrast-to-noise ratio (CNR) and to enlarge the field of view (FOV). We demonstrate experimentally high CNR subdiffraction imaging with lateral resolution of 122 nm and FOV of thousands of micrometers square.
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http://dx.doi.org/10.1364/OL.42.004569 | DOI Listing |
High-performance polarization-multiplexed metalenses (PMMs) hold remarkable transformative potential in optical platforms. However, balancing the numerical aperture (NA), focusing efficiency, and spectral bandwidth remains a significant challenge in the existing PMMs, thus restricting their extensive applications. To circumvent these challenges, we theoretically demonstrate two unique orthogonal (linear and circular) PMMs based on all 4H-silicon carbide (4H-SiC) with superior NA, sound focusing efficiency, and broad wavelength range.
View Article and Find Full Text PDFWe introduce super-resolution panoramic integration (SPI), an on-the-fly microscopy technique enabling instantaneous generation of subdiffractional images concurrently with scalable, high-throughput screening. SPI leverages multifocal optical rescaling, high-content sweeping, and synchronized line-scan readout while preserving minimal post-processing and compatibility with epi-fluorescence settings. We demonstrate SPI for various subcellular and populational morphology, function, and heterogeneity.
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August 2025
Zhejiang Key Laboratory of Functional Structural Lipid Synthesis and Application, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China.
A unique class of bioorthogonal fluorogenic probes, based on difluoroboronated tetrazine (), has been developed. These probes are the smallest tetrazine-based fluorescent structures and exhibit a high fluorescence turn-on ratio (up to 582-fold) following the inverse electron-demand Diels-Alder reaction. By engineering π-conjugated systems and using push-pull groups, their fluorescence emission can be tuned across blue to yellow (440 to 570 nanometers).
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March 2025
Breaking the diffraction limit has been a key challenge in optical engineering and super-resolution imaging. In this work, we utilize a vectorial Debye integral neural network to design sub-diffraction focusing fields for high-NA objectives. By training the polarization states of incident light, we flexibly achieve transitions from diffraction-limited focusing to superoscillatory regimes.
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March 2025
Planar diffractive lenses (PDLs) based on the principle of optical superoscillation, have revolutionized far-field super-resolution imaging. A plethora of versatile PDLs have been designed for the visible wavelengths. However, it is challenging to realize PDLs in the vacuum ultraviolet (VUV) band.
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