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http://dx.doi.org/10.18632/aging.101314 | DOI Listing |
mBio
April 2025
Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia.
Unlabelled: Type II toxin-antitoxin (TA) systems are widespread in prokaryotes. They consist of neighboring genes encoding two small proteins: a toxin that inhibits a critical cellular process and an antitoxin that binds to and neutralizes the toxin. The VapD nuclease and the VapX antitoxin comprise a type II TA system that contributes to the virulence of the human pathogen .
View Article and Find Full Text PDFJ Phys Condens Matter
February 2025
UGC DAE Consortium for Scientific Research, Indore 452001, India.
Coupling between different interactions allows the control of physical aspects in multifunctional materials by perturbing any degrees of freedom. Here, we aim to probe the correlation among structural, electronic, and magnetic observables in SmNiMnO(SNMO) ferromagnetic insulator double perovskite. Our employed methodology includes thermal evolution of x-ray diffraction, x-ray absorption spectroscopy, and bulk magnetometry.
View Article and Find Full Text PDFJ Biol Chem
May 2024
Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, USA; Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, USA; Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, USA; Department
Over the past 3 decades, a diverse collection of small protein domains have been used as scaffolds to generate general purpose protein-binding reagents using a variety of protein display and enrichment technologies. To expand the repertoire of scaffolds and protein surfaces that might serve this purpose, we have explored the utility of (i) a pair of anti-parallel alpha-helices in a small highly disulfide-bonded 4-helix bundle, the CC4 domain from reversion-inducing Cysteine-rich Protein with Kazal Motifs and (ii) a concave beta-sheet surface and two adjacent loops in the human FN3 domain, the scaffold for the widely used monobody platform. Using M13 phage display and next generation sequencing, we observe that, in both systems, libraries of ∼30 million variants contain binding proteins with affinities in the low μM range for baits corresponding to the extracellular domains of multiple mammalian proteins.
View Article and Find Full Text PDFNat Commun
December 2023
Department of Food Science and Biotechnology, National Chung Hsing University, Taichung, 402202, Taiwan.
The cyclic GMP-AMP synthase (cGAS)/stimulator of interferon gene (STING) signaling pathway plays a critical protective role against viral infections. Metazoan STING undergoes multilayers of regulation to ensure specific signal transduction. However, the mechanisms underlying the regulation of bacterial STING remain unclear.
View Article and Find Full Text PDFFront Mol Biosci
September 2023
Department of Pharmacology, University of Minnesota Medical School, Minneapolis, MN, United States.
Cellular retinoic acid (RA)-binding protein 1 (CRABP1) is a highly conserved protein comprised of an anti-parallel, beta-barrel, and a helix-turn-helix segment outside this barrel. Functionally, CRABP1 is thought to bind and sequester cytosolic RA. Recently, CRABP1 has been established as a major mediator of rapid, non-genomic activity of RA in the cytosol, referred to as "non-canonical" activity.
View Article and Find Full Text PDF